Pentoxifylline/Valsartan co-delivery in liposomal gel alters the inflammatory HMGB-1/ TLR pathway and promotes faster healing in burn wounds: A promising repurposed approach

• Published in: International journal of pharmaceutics 2022-09, Vol.625, p.122129-122129, Article 122129
• Main Authors: El-Salamouni, Noha S., Gowayed, Mennatallah A., Younis, Sameh E., Abdel-Bary, Ahmed, Kamel, Maher A., Labib, Gihan S.
• Format: Article
• Language: English
• Subjects: Liposomes; Pentoxifylline; Topical; Ulcer; Valsartan; Wound healing
• ISSN: 0378-5173; 1873-3476
• DOI: 10.1016/j.ijpharm.2022.122129

[Display omitted] •The effect of PTX and VAL simultaneously in a nanometric liposomal delivery system for the treatment of burn ulcer.•Innovation in multiloading liposomal stable drug delivery system with two drugs of different classes with appropriate entrapment efficiency.•The pharmacological mechanism of a combined therapy in ulcer management.•A new simple, economic and reliable validated spectrophotometric method for the co– analysis of PTX and VAL in the final delivery product was also developed. Burn wounds are one of the most severe complex forms of trauma. Hence, new treatment strategies that facilitate the healing process; reduce the severity and the healing time is the main concern of the health care systems. In this work, pentoxifylline-valsartan, (PTX- VAL), loaded liposomes integrated into gel were designed for the first time as a novel co-delivery carrier for the treatment of burn wounds. The objective of this work was to investigate the ability of the nano-based liposomal system to co-entrap two repurposed drugs; hydrophilic pentoxifylline and lipophilic valsartan for topical treatment of burn wounds. The impact of increasing the phospholipid amount to enhance the co-entrapment of PTX and VAL was investigated and in-vitro evaluation of the prepared formulations was conducted to choose the optimum composition with the highest entrapment of both drugs adopting a simple, reliable derivative spectrophotometric method. Structure elucidation was also performed using a transmission electron microscope. In addition, A simple selected derivative spectrophotometric method was developed for the assay of PTX-VAL novel combination. The proven selectivity, precision and accuracy assured the reliability of this analytical method. Being economic and fast makes routine application of the developed analytical method is recommended in pharmaceutical industry. The selected liposomal formulation integrated into gel matrix (PTX-VAL-LG) showed; nanometric size, acceptable entrapment efficiency of both PTX and VAL as well as sustained release profiles and thus, enhanced action.