Molecular detection and patho-morphological study of enteric Escherichia coli pathotypes in diarrheic neonatal calves

To understand the pathology of natural cases of E. coli pathotypes infection in bovine calves, 45 cases of bovine calves, below one month of age, died due to enteritis were studied. Total seventeen cases (37.77%) turned positive for different pathotypes of E. coli by RT-PCR. Out of seventeen positiv...

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Bibliographic Details
Published in:Animal biotechnology 2023-12, Vol.34 (7), p.3267-3273
Main Authors: Singh, Shailendra, Singh, Rajendra, Singh, Karam P., Singh, Rahul, Kumar, Pawan, Kamdi, Bhupesh, Singh, Vidhya
Format: Article
Language:English
Subjects:
Calves
Cattle
Crypts
Duodenum
E coli
eae gene
Enteritis
FimA gene
Follicles
Hemorrhage
Histopathology
Ileum
Intestine
Jejunum
Lamina propria
Lesions
Leukocytes (mononuclear)
Lymph nodes
Lymphatic system
Mucosa
Necrosis
Neonates
Sinuses
Small intestine
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Summary:To understand the pathology of natural cases of E. coli pathotypes infection in bovine calves, 45 cases of bovine calves, below one month of age, died due to enteritis were studied. Total seventeen cases (37.77%) turned positive for different pathotypes of E. coli by RT-PCR. Out of seventeen positive samples for E. coli, six cases (35.29%) were positive for eae gene, three cases (17.64%) for bfp gene and eight cases (47.05) for fimA gene of E. coli. Gross lesions in these cases showed pin-point to ecchymotic hemorrhages in the mucosa of jejunum, ileum and colon. The draining mesenteric lymph nodes were swollen, enlarged and showed cord -like structure. Histopathology of small intestine showed, villi lining cells were sloughed off, tips of villi capillary plexus were congested and hemorrhagic, and skipping lesions of microabscesses in the crypts of mucosa were observed. In the duodenum, necrosis of crypts and infiltration of mononuclear cells in the lamina propria and around Brunner’s gland. In mesenteric lymph nodes the subscapular space were infiltrated with mononuclear cells with depletion of lymphoid follicles in cortical area. Peri-trabecular and medullary sinuses of mesenteric lymph nodes were necrosed.
ISSN:1049-5398
1532-2378
DOI:10.1080/10495398.2022.2114003