Seed dormancy 4 like1 of Arabidopsis is a key regulator of phase transition from embryo to vegetative development

• Published in: The Plant journal : for cell and molecular biology 2022-10, Vol.112 (2), p.460-475
• Main Authors: Zheng, Lipeng, Otani, Masahiko, Kanno, Yuri, Seo, Mitsunori, Yoshitake, Yushi, Yoshimoto, Kohki, Sugimoto, Kazuhiko, Kawakami, Naoto
• Format: Article
• Language: English
• Subjects: Arabidopsis; Arabidopsis thaliana; Crop production; DOG1; Dormancy; Drying; Embryos; Food quality; Gene mapping; Germination; LAFL; Maturation; Mutants; phase transition; Phase transitions; Phenotypes; precocious germination; preharvest sprouting; Quantitative trait loci; Rice; Sdr4; seed; seedling establishment; Seedlings; Seeds
• ISSN: 0960-7412; 1365-313X
• DOI: 10.1111/tpj.15959

SUMMARY Seed dormancy is an adaptive trait that enables plants to survive adverse conditions and restart growth in a season and location suitable for vegetative and reproductive growth. Control of seed dormancy is also important for crop production and food quality because it can help induce uniform germination and prevent preharvest sprouting. Rice preharvest sprouting quantitative trait locus analysis has identified Seed dormancy 4 (Sdr4) as a positive regulator of dormancy development. Here, we analyzed the loss‐of‐function mutant of the Arabidopsis ortholog, Sdr4 Like1 (SFL1), and found that the sfl1‐1 seeds showed precocious germination at the mid‐ to late‐maturation stage similar to rice sdr4 mutant, but converted to become more dormant than the wild type during maturation drying. Coordinated with the dormancy levels, expression levels of the seed maturation and dormancy master regulator genes, ABI3, FUS3, and DOG1 in sfl1‐1 seeds were lower than in wild type at early‐ and mid‐maturation stages, but higher at the late‐maturation stage. In addition to the seed dormancy phenotype, sfl1‐1 seedlings showed a growth arrest phenotype and heterochronic expression of LAFL (LEC1, ABI3, FUS3, LEC2) and DOG1 in the seedlings. These data suggest that SFL1 is a positive regulator of initiation and termination of the seed dormancy program. We also found genetic interaction between SFL1 and the SFL2, SFL3, and SFL4 paralogs of SFL1, which impacts on the timing of the phase transition from embryo maturation to seedling growth. Significance Statement Seed dormancy is an adaptive trait for wild species and an agronomically important trait for cultivated species. We found that the Arabidopsis ortholog of rice Sdr4, SFL1, positively regulates the initiation and termination of the seed dormancy program. Our data suggest functional conversion of SFL1 from a positive to a negative feedback regulator for expression of the seed maturation master regulator genes, LAFL, which accomplish phase transition from embryo to vegetative development.