Effect of alcohol on productivity and quality of adeno-associated virus 2 in HEK293 cells

• Published in: Journal of bioscience and bioengineering 2022-10, Vol.134 (4), p.338-347
• Main Authors: Shiina, Shunsuke, Nagao, Nobuyoshi, Hasegawa, Junichi, Sato, Tori, Mori, Chinatsu, Ohtaki, Kaya, Kubodera, Kiyomi, Yamashita, Yuri, Tanabe, Kana, Kawano, Yasuhiro, Aoyagi, Hideki
• Format: Article
• Language: English
• Subjects: Adeno associated virus vector; Pathway analysis; Production enhancer; Proteome analysis; Suspension HEK293 cells culture
• ISSN: 1389-1723; 1347-4421
• DOI: 10.1016/j.jbiosc.2022.07.006

Investigation of enhancers to improve recombinant adeno-associated virus 2 (rAAV2) productivity by human embryonic kidney 293 cells (HEK293) suspension culture showed that the addition of ethanol improved the productivity and packaged genome integrity of rAAV2. Further optimization showed that adding ethanol in the range of 0.09%–1.11% (v/v) during rAAV2 production effectively improved rAAV2 productivity and quality. In addition, ethanol addition improved cell viability. Furthermore, proteome and pathway analysis of the cells during rAAV2 production showed that the addition of ethanol resulted in the upregulation of pathways related to intercellular signaling, gene expression, cell morphology, intercellular maintenance, and others. In contrast, pathways related to cell death, immunity, and reactions to infection were downregulated. These changes in pathway regulation were responsible for the improvement in rAAV2 productivity, packaged genome integrity, and cell viability during rAAV2 production. The results of this study can be applied to the production of viral vectors for in vivo gene therapy in an inexpensive and safe manner. •VPA in ethanol increases the productivity and genomic integrity of rAAV2 produced by HEK293 cells.•Effect is due to ethanol; VPA itself has a inhibitory effect•Ethanol also increases producer cell viability.•Low concentrations of ethanol (