Research Development on Exosome Separation Technology

Exosomes are special extracellular vesicles secreted by cells, which are of great significance in the basic research of life science and clinical application and has become a hot research field with rapid development in recent 10 years. Therefore, the isolation and separation of exosomes is particul...

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Published in:The Journal of membrane biology 2023-02, Vol.256 (1), p.25-34
Main Authors: Xu, Wei-Ming, Li, Ao, Chen, Jia-Jun, Sun, En-Jie
Format: Article
Language:English
Subjects:
Aptamers
Biochemistry
Biological Transport
Biology
Biomarkers
Biomedical and Life Sciences
Cell culture
Chromatography
Exosomes
Exosomes - chemistry
Exosomes - metabolism
Extracellular Vesicles
Human Physiology
Life Sciences
Membrane filters
Methods
Microfluidics
Plasma
Proteins
R&D
Research & development
Separation
Ultracentrifugation
Ultracentrifugation - methods
Ultrafiltration
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creator Xu, Wei-Ming
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description Exosomes are special extracellular vesicles secreted by cells, which are of great significance in the basic research of life science and clinical application and has become a hot research field with rapid development in recent 10 years. Therefore, the isolation and separation of exosomes is particularly important for the research and application of exosomes. This paper aims to review the research progress of exosome isolation and separation methods in recent years, including ultracentrifugation, ultrafiltration, size‑exclusion chromatography, precipitation, immunomagnetic bead capture method, aptamer-based isolation, and isolation methods based on microfluidic technology. It is generally accepted that most of the existing methods have limitations, for example, ultracentrifugation is time-consuming and laborious, and immunomagnetic bead capture method and aptamer-based separation method have small sample processing capacity and high cost. As a result, we also introduce some common situations in which two or more methods are combined for use. Finally, the separation and isolation methods including all those presented in this review were compared and summarized. Graphical Abstract
doi_str_mv 10.1007/s00232-022-00260-y
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subjects Aptamers
Biochemistry
Biological Transport
Biology
Biomarkers
Biomedical and Life Sciences
Cell culture
Chromatography
Exosomes
Exosomes - chemistry
Exosomes - metabolism
Extracellular Vesicles
Human Physiology
Life Sciences
Membrane filters
Methods
Microfluidics
Plasma
Proteins
R&D
Research & development
Separation
Ultracentrifugation
Ultracentrifugation - methods
Ultrafiltration
title Research Development on Exosome Separation Technology
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