Influence of exogenous phytohormone supplementation on the pigment and fatty acid content of three marine diatoms

• Published in: Applied microbiology and biotechnology 2022-09, Vol.106 (18), p.6195-6207
• Main Authors: Fierli, David, Aranyos, Anita, Barone, Maria Elena, Parkes, Rachel, Touzet, Nicolas
• Format: Article
• Language: English
• Subjects: Abscisic acid; Algae; Analysis; Applied Microbial and Cell Physiology; Aquatic microorganisms; Bacillariophyceae; Biomedical and Life Sciences; Biotechnology; Carotene; Commercialization; Diatoms; Eicosapentaenoic acid; Fatty acids; Food and nutrition; Fucoxanthin; Gibberellic acid; Growth regulators; Identification and classification; Indoleacetic acid; Influence; Life Sciences; Lipids; Marine microorganisms; Methyl jasmonate; Microalgae; Microbial Genetics and Genomics; Microbiology; Microorganisms; Nitzschia; Optimization; Photosynthesis; Physiological aspects; Phytohormones; Pigments; Plankton; Plant growth; Plant hormones; Plant pigments; Production costs; Salicylic acid; Species; Stauroneis; Supplements; β-Carotene
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-022-12140-5

Diatoms are ubiquitous photosynthetic microorganisms with great potential for biotechnological applications. However, their commercialisation is hampered by production costs, requiring hence optimisation of cultivation methods. Phytohormones are plant growth regulators which may be used to influence physiological processes in microalgae, including diatoms. In this study, the model species Phaeodactylum tricornutum ( Phaeodactylaceae ) and two Irish isolates of Stauroneis sp. ( Stauroneidaceae ) and Nitzschia sp. ( Bacillariaceae ) were grown with varying amounts of the phytohormones indoleacetic acid (IAA), gibberellic acid (GA3), methyl jasmonate (MJ), abscisic acid (ABA) or salicylic acid (SA), and their influence on pigment and fatty acid profiles was monitored. The application of GA3 (200 mg/l) stimulated the growth of P. tricornutum which accumulated 52% more dry biomass compared to the control and concomitantly returned the highest eicosapentaenoic acid (EPA) yield (0.6 mg/l). The highest fucoxanthin yield (0.18 mg/l) was obtained for P. tricornutum cultivated with GA3 (2 mg/l) supplementation. In Stauroneis sp., SA (1 mg/l) had the most positive effect on EPA, the content of which was enhanced up to 45.7 μg/mg (4.6% of total dry weight). The SA (1 mg/l) treatment also boosted carotenogenesis in Nitzschia sp., leading to 1.7- and 14-fold increases in fucoxanthin and β-carotene compared to the control, respectively. Of note, MJ (0.5 mg/l) increased the EPA content of all diatom species compared to their controls. These results indicate that phytohormone-based treatments can be used to alter the pigment and lipid content of microalgae, which tend to respond in dose- and species-specific manners to individual compounds. Key points • Response to phytohormones was investigated in diatoms from distinct families . • MJ (0.5 mg/l) caused an increase in EPA cellular content in all three diatoms . • Phytohormones mostly caused dose-dependent and species-specific responses .