Nano-gold-enhanced LAMP method for qualitative visual detection of Salmonella in milk

• Published in: Mikrochimica acta (1966) 2022-09, Vol.189 (9), p.365-365, Article 365
• Main Authors: Du, ZaiHui, Lin, ShengHao, Li, JiaLe, Tian, JingJing, Xu, WenTao, Huang, KunLun, Liu, QingLiang, Sun, YanLi
• Format: Article
• Language: English
• Subjects: Analytical Chemistry; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Food; Gold; Health aspects; Liquid phases; Methods; Microengineering; Milk; Nanochemistry; Nanotechnology; Original Paper; Public health; Reaction time; Safety and security measures; Salmonella; Salmonella food poisoning
• ISSN: 0026-3672; 1436-5073
• DOI: 10.1007/s00604-022-05459-3

Since Salmonella can cause foodborne disease and public health safety issues and requires a robust, rapid, on-site detection method, a novel visual qualitative method with nano-gold-enhanced loop-mediated isothermal amplification (LAMP) reaction was established for detecting Salmonella in an integrated tube. During the experiment, nano-gold were used to enhance LAMP amplification, improving amplification efficiency and shortening the reaction time to within 30 min. Visual qualitative detection is achieved via negative staining, involving the addition of CuSO 4 to the final products of the LAMP reaction. Ring-like white accumulation occurs in the absence of Salmone lla targets but not when they are present. After completing the LAMP reaction, the integration tube was shaken gently for 1 min to observe the liquid phase system changes, realizing the closed tube detection of Salmonella . The process resolved the challenge presented by cross-contamination, false positives, and nonspecific amplification during the LAMP reaction. This method was used to detect Salmonella in milk, further highlighting its prospects in the field of rapid food safety detection. Graphical abstract