Developmental validation of the Microreader Group Y Direct ID System: A novel six‐dye typing system with 54 Y‐chromosomal loci for forensic application

Y‐chromosome, as a gender‐determined biological marker, is inherited only between fathers and sons. The Y‐chromosome short tandem repeats (Y‐STRs) play an essential role in paternity lineage tracing as well as sexual assault cases. The Microreader Group Y Direct ID System as a six‐dye multiplex ampl...

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Bibliographic Details
Published in:Electrophoresis 2022-10, Vol.43 (20), p.2023-2032
Main Authors: Song, Mengyuan, Jiang, Lanrui, Lyu, Qiang, Ying, Jun, Wang, Zefei, Zhou, Yuxiang, Song, Feng, Luo, Haibo, Song, Xingbo, Ying, Binwu
Format: Article
Language:English
Subjects:
Biomarkers
China
Chromosomes
Chromosomes, Human, Y - genetics
DNA Fingerprinting
Dyes
Female
forensic genetics
Haplotypes
Humans
Male
Males
Microreader Group Y Direct ID System
Microsatellite Repeats - genetics
Paternity
Performance enhancement
Polymorphism
Reproducibility of Results
Sex crimes
validation studies
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Summary:Y‐chromosome, as a gender‐determined biological marker, is inherited only between fathers and sons. The Y‐chromosome short tandem repeats (Y‐STRs) play an essential role in paternity lineage tracing as well as sexual assault cases. The Microreader Group Y Direct ID System as a six‐dye multiplex amplification kit, including 53 Y‐STR and one Y‐Indel locus, would improve performance and aid in obtaining more information through a greater number of loci with high polymorphism. In the present study, to verify the accuracy and efficiency of the kit, developmental validation was conducted by investigating sensitivity, species specificity, PCR inhibition, male–male and male–female mixtures, and reproducibility. The kit was tested using 311 male samples from Han and Qiang populations in Sichuan Province. The results showed that this kit had fairly high power for forensic discrimination (Han: haplotype diversity [HD] = 1, Qiang: HD = 0.999944). Additionally, 44 confirmed father–son pairs were also genotyped, among which 69 distinct haplotypes could be obtained. These father–son pairs cannot be distinguished by commonly used Y‐STR panels, indicating that adding these extra Y‐STRs to a single panel can achieve better discrimination performance. Collectively, the Microreader Group Y Direct ID System is robust and informative for forensic applications.
ISSN:0173-0835
1522-2683
DOI:10.1002/elps.202200109