Transcriptomic Analysis of DNA Repair Pathways in Human Non-Small Cell Lung Cancer Cells Surviving Multifraction X-Ray Irradiation

Radioresistant sublines of non-small cell lung cancer cells differing in the p53 status were obtained: A549 (p53 wild type) and H1299 (p53 deficient). The exposure to ionizing radiation was carried out using a standard protocol developed on the basis of empirical clinical experience and consisting i...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2022-08, Vol.173 (4), p.454-458
Main Authors: Pustovalova, M. V., Guryanova, A. A., Sorokin, M. I., Suntsova, M. V., Buzdin, A. A., Alhaddad, L., Osipov, A. N., Leonov, S. V.
Format: Article
Language:English
Subjects:
Analysis
Biomedical and Life Sciences
Biomedicine
Cancer
Cancer cells
Cancer therapies
Cell Biology
Cell culture
DNA
DNA repair
Gene expression
Genes
Genetic aspects
Internal Medicine
Ionizing radiation
Laboratory Medicine
Lung cancer
Lung cancer, Non-small cell
Lung cancer, Small cell
Non-small cell lung carcinoma
p53 Protein
Pathology
Radiation
Radiation therapy
Radiosensitivity
Small cell lung carcinoma
Software
Transcriptomics
Tumor proteins
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Summary:Radioresistant sublines of non-small cell lung cancer cells differing in the p53 status were obtained: A549 (p53 wild type) and H1299 (p53 deficient). The exposure to ionizing radiation was carried out using a standard protocol developed on the basis of empirical clinical experience and consisting in exposure in a dose of 2 Gy once a day, 5 days a week up to total dose of 60 Gy. The cells survived after irradiation demonstrated reduced radiosensitivity, as well as changes in differential gene expression in comparison with parental cells. Some differences in the signaling pathways involved in DNA repair were revealed.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-022-05586-0