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Low expression of isocitrate dehydrogenase 1 (IDH1) R132H is associated with advanced pathological features in laryngeal squamous cell carcinoma
Introduction Recent developments in genomic sequencing have led to the identification of somatic mutations in isocitrate dehydrogenase 1 (IDH1) in various malignancies. IDH1 R132H is the most common mutation of IDH1, which affects codon 132 and results in the conversion of amino acid residue arginin...
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| Published in: | Journal of cancer research and clinical oncology 2023-07, Vol.149 (8), p.4253-4267 |
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| container_title | Journal of cancer research and clinical oncology |
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| creator | Shayanfar, Nasrin Zare-Mirzaie, Ali Mohammadpour, Mahsa Jafari, Ensieh Mehrtash, Amirhosein Emtiazi, Nikoo Tajik, Fatemeh |
| description | Introduction
Recent developments in genomic sequencing have led to the identification of somatic mutations in isocitrate dehydrogenase 1 (IDH1) in various malignancies. IDH1 R132H is the most common mutation of IDH1, which affects codon 132 and results in the conversion of amino acid residue arginine (R) to histidine (H). This study is designed to evaluate the association between the expression of IDH1 R132H and clinicopathological characteristics in laryngeal squamous cell carcinoma (LSCC).
Methods
The expression pattern and clinical significance of IDH1 R132H were investigated in tissue microarrays (TMAs) of 50 LSCC tumors as well as adjacent normal tissues using immunohistochemistry. Then the exons of the 12 tumor samples with negative/weak positive staining were sequenced by applying polymerase chain reaction (PCR).
Results
The results demonstrated that the cytoplasmic expression of IDH1 R132H was downregulated in tumor cells compared to adjacent normal tissues. A statistically significant association was found between a low level of cytoplasmic expression of IDH1 R132H protein and an increase in histological grade (
p
|
| doi_str_mv | 10.1007/s00432-022-04336-z |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2709914807</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2837642725</sourcerecordid><originalsourceid>FETCH-LOGICAL-c419t-d1837bcdb5b1aca7a61506a91d45570f9307ba0c835a5350f90438d97443c9683</originalsourceid><addsrcrecordid>eNp9kU1r3DAQhkVoaTZp_0AORZBLcnCjD8uyjyX92MBCoaRnMZbkXQXb2kh2vn5Ff3Jnu2kLOfQgxIyemXk1LyEnnH3gjOmLzFgpRcEEnlLKqng6IAu-S3Ep1SuyYFzzQgleHZKjnG8YxkqLN-RQVqySQogF-bmK99Q_bJPPOcSRxo6GHG2YEkyeOr95dCmu_QjZU07Prj4t-Tn9zqVYIkch71gkHb0P04aCu4PRYrSFaRP7uA4Wetp5mGYcQMNIe0iP49pjNt_OMMQ5U-v7nlpINoxxgLfkdQd99u-e72Py48vn68tlsfr29ery46qwJW-mwvFa6ta6VrUcLGiouGIVNNyVSmnWNZLpFpitpQIlFSZwRbVrdFlK21S1PCZn-77bFG9nnyczhLyTAqNHVUZo1jS8rJlG9PQFehPnNKI6I1BFVQotFFJiT9kUc06-M9sUBvyu4czs_DJ7vwz6ZX77ZZ6w6P1z67kdvPtb8scgBOQeyPiEi0v_Zv-n7S87v6DO</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2837642725</pqid></control><display><type>article</type><title>Low expression of isocitrate dehydrogenase 1 (IDH1) R132H is associated with advanced pathological features in laryngeal squamous cell carcinoma</title><source>Springer Link</source><creator>Shayanfar, Nasrin ; Zare-Mirzaie, Ali ; Mohammadpour, Mahsa ; Jafari, Ensieh ; Mehrtash, Amirhosein ; Emtiazi, Nikoo ; Tajik, Fatemeh</creator><creatorcontrib>Shayanfar, Nasrin ; Zare-Mirzaie, Ali ; Mohammadpour, Mahsa ; Jafari, Ensieh ; Mehrtash, Amirhosein ; Emtiazi, Nikoo ; Tajik, Fatemeh</creatorcontrib><description>Introduction
Recent developments in genomic sequencing have led to the identification of somatic mutations in isocitrate dehydrogenase 1 (IDH1) in various malignancies. IDH1 R132H is the most common mutation of IDH1, which affects codon 132 and results in the conversion of amino acid residue arginine (R) to histidine (H). This study is designed to evaluate the association between the expression of IDH1 R132H and clinicopathological characteristics in laryngeal squamous cell carcinoma (LSCC).
Methods
The expression pattern and clinical significance of IDH1 R132H were investigated in tissue microarrays (TMAs) of 50 LSCC tumors as well as adjacent normal tissues using immunohistochemistry. Then the exons of the 12 tumor samples with negative/weak positive staining were sequenced by applying polymerase chain reaction (PCR).
Results
The results demonstrated that the cytoplasmic expression of IDH1 R132H was downregulated in tumor cells compared to adjacent normal tissues. A statistically significant association was found between a low level of cytoplasmic expression of IDH1 R132H protein and an increase in histological grade (
p
< 0.001), perineural invasion (
p
= 0.019), and lymph node involvement (
p
< 0.001). The exon4 sequencing results showed that only one sample was positive for IDH1 R132H mutation. IDH1 R132H expression was observed in 39 (78.0%) LSCC samples.
Conclusion
These findings indicate that low cytoplasmic expression of IDH1 R132H may have clinical significance in LSCC patients and is associated with more aggressive tumor behavior and progression of the disease, which can help improve potential treatment in patients with LSCC. Further investigations are needed to understand the biological function of IDH1 R132H and larger sample size to confirm our findings.</description><identifier>ISSN: 0171-5216</identifier><identifier>EISSN: 1432-1335</identifier><identifier>DOI: 10.1007/s00432-022-04336-z</identifier><identifier>PMID: 36063222</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Amino acids ; Cancer Research ; Clinical significance ; Dehydrogenases ; Exons ; Hematology ; Histidine ; Immunohistochemistry ; Internal Medicine ; Isocitrate dehydrogenase ; Laryngeal cancer ; Laryngeal carcinoma ; Lymph nodes ; Malignancy ; Medicine ; Medicine & Public Health ; Mutation ; Oncology ; Patients ; Polymerase chain reaction ; Squamous cell carcinoma ; Statistical analysis ; Tumor cells ; Tumors</subject><ispartof>Journal of cancer research and clinical oncology, 2023-07, Vol.149 (8), p.4253-4267</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022. Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c419t-d1837bcdb5b1aca7a61506a91d45570f9307ba0c835a5350f90438d97443c9683</citedby><cites>FETCH-LOGICAL-c419t-d1837bcdb5b1aca7a61506a91d45570f9307ba0c835a5350f90438d97443c9683</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36063222$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shayanfar, Nasrin</creatorcontrib><creatorcontrib>Zare-Mirzaie, Ali</creatorcontrib><creatorcontrib>Mohammadpour, Mahsa</creatorcontrib><creatorcontrib>Jafari, Ensieh</creatorcontrib><creatorcontrib>Mehrtash, Amirhosein</creatorcontrib><creatorcontrib>Emtiazi, Nikoo</creatorcontrib><creatorcontrib>Tajik, Fatemeh</creatorcontrib><title>Low expression of isocitrate dehydrogenase 1 (IDH1) R132H is associated with advanced pathological features in laryngeal squamous cell carcinoma</title><title>Journal of cancer research and clinical oncology</title><addtitle>J Cancer Res Clin Oncol</addtitle><addtitle>J Cancer Res Clin Oncol</addtitle><description>Introduction
Recent developments in genomic sequencing have led to the identification of somatic mutations in isocitrate dehydrogenase 1 (IDH1) in various malignancies. IDH1 R132H is the most common mutation of IDH1, which affects codon 132 and results in the conversion of amino acid residue arginine (R) to histidine (H). This study is designed to evaluate the association between the expression of IDH1 R132H and clinicopathological characteristics in laryngeal squamous cell carcinoma (LSCC).
Methods
The expression pattern and clinical significance of IDH1 R132H were investigated in tissue microarrays (TMAs) of 50 LSCC tumors as well as adjacent normal tissues using immunohistochemistry. Then the exons of the 12 tumor samples with negative/weak positive staining were sequenced by applying polymerase chain reaction (PCR).
Results
The results demonstrated that the cytoplasmic expression of IDH1 R132H was downregulated in tumor cells compared to adjacent normal tissues. A statistically significant association was found between a low level of cytoplasmic expression of IDH1 R132H protein and an increase in histological grade (
p
< 0.001), perineural invasion (
p
= 0.019), and lymph node involvement (
p
< 0.001). The exon4 sequencing results showed that only one sample was positive for IDH1 R132H mutation. IDH1 R132H expression was observed in 39 (78.0%) LSCC samples.
Conclusion
These findings indicate that low cytoplasmic expression of IDH1 R132H may have clinical significance in LSCC patients and is associated with more aggressive tumor behavior and progression of the disease, which can help improve potential treatment in patients with LSCC. Further investigations are needed to understand the biological function of IDH1 R132H and larger sample size to confirm our findings.</description><subject>Amino acids</subject><subject>Cancer Research</subject><subject>Clinical significance</subject><subject>Dehydrogenases</subject><subject>Exons</subject><subject>Hematology</subject><subject>Histidine</subject><subject>Immunohistochemistry</subject><subject>Internal Medicine</subject><subject>Isocitrate dehydrogenase</subject><subject>Laryngeal cancer</subject><subject>Laryngeal carcinoma</subject><subject>Lymph nodes</subject><subject>Malignancy</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Mutation</subject><subject>Oncology</subject><subject>Patients</subject><subject>Polymerase chain reaction</subject><subject>Squamous cell carcinoma</subject><subject>Statistical analysis</subject><subject>Tumor cells</subject><subject>Tumors</subject><issn>0171-5216</issn><issn>1432-1335</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNp9kU1r3DAQhkVoaTZp_0AORZBLcnCjD8uyjyX92MBCoaRnMZbkXQXb2kh2vn5Ff3Jnu2kLOfQgxIyemXk1LyEnnH3gjOmLzFgpRcEEnlLKqng6IAu-S3Ep1SuyYFzzQgleHZKjnG8YxkqLN-RQVqySQogF-bmK99Q_bJPPOcSRxo6GHG2YEkyeOr95dCmu_QjZU07Prj4t-Tn9zqVYIkch71gkHb0P04aCu4PRYrSFaRP7uA4Wetp5mGYcQMNIe0iP49pjNt_OMMQ5U-v7nlpINoxxgLfkdQd99u-e72Py48vn68tlsfr29ery46qwJW-mwvFa6ta6VrUcLGiouGIVNNyVSmnWNZLpFpitpQIlFSZwRbVrdFlK21S1PCZn-77bFG9nnyczhLyTAqNHVUZo1jS8rJlG9PQFehPnNKI6I1BFVQotFFJiT9kUc06-M9sUBvyu4czs_DJ7vwz6ZX77ZZ6w6P1z67kdvPtb8scgBOQeyPiEi0v_Zv-n7S87v6DO</recordid><startdate>20230701</startdate><enddate>20230701</enddate><creator>Shayanfar, Nasrin</creator><creator>Zare-Mirzaie, Ali</creator><creator>Mohammadpour, Mahsa</creator><creator>Jafari, Ensieh</creator><creator>Mehrtash, Amirhosein</creator><creator>Emtiazi, Nikoo</creator><creator>Tajik, Fatemeh</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20230701</creationdate><title>Low expression of isocitrate dehydrogenase 1 (IDH1) R132H is associated with advanced pathological features in laryngeal squamous cell carcinoma</title><author>Shayanfar, Nasrin ; Zare-Mirzaie, Ali ; Mohammadpour, Mahsa ; Jafari, Ensieh ; Mehrtash, Amirhosein ; Emtiazi, Nikoo ; Tajik, Fatemeh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c419t-d1837bcdb5b1aca7a61506a91d45570f9307ba0c835a5350f90438d97443c9683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Amino acids</topic><topic>Cancer Research</topic><topic>Clinical significance</topic><topic>Dehydrogenases</topic><topic>Exons</topic><topic>Hematology</topic><topic>Histidine</topic><topic>Immunohistochemistry</topic><topic>Internal Medicine</topic><topic>Isocitrate dehydrogenase</topic><topic>Laryngeal cancer</topic><topic>Laryngeal carcinoma</topic><topic>Lymph nodes</topic><topic>Malignancy</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Mutation</topic><topic>Oncology</topic><topic>Patients</topic><topic>Polymerase chain reaction</topic><topic>Squamous cell carcinoma</topic><topic>Statistical analysis</topic><topic>Tumor cells</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shayanfar, Nasrin</creatorcontrib><creatorcontrib>Zare-Mirzaie, Ali</creatorcontrib><creatorcontrib>Mohammadpour, Mahsa</creatorcontrib><creatorcontrib>Jafari, Ensieh</creatorcontrib><creatorcontrib>Mehrtash, Amirhosein</creatorcontrib><creatorcontrib>Emtiazi, Nikoo</creatorcontrib><creatorcontrib>Tajik, Fatemeh</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest Public Health Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Research Library</collection><collection>Research Library (Corporate)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cancer research and clinical oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shayanfar, Nasrin</au><au>Zare-Mirzaie, Ali</au><au>Mohammadpour, Mahsa</au><au>Jafari, Ensieh</au><au>Mehrtash, Amirhosein</au><au>Emtiazi, Nikoo</au><au>Tajik, Fatemeh</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Low expression of isocitrate dehydrogenase 1 (IDH1) R132H is associated with advanced pathological features in laryngeal squamous cell carcinoma</atitle><jtitle>Journal of cancer research and clinical oncology</jtitle><stitle>J Cancer Res Clin Oncol</stitle><addtitle>J Cancer Res Clin Oncol</addtitle><date>2023-07-01</date><risdate>2023</risdate><volume>149</volume><issue>8</issue><spage>4253</spage><epage>4267</epage><pages>4253-4267</pages><issn>0171-5216</issn><eissn>1432-1335</eissn><abstract>Introduction
Recent developments in genomic sequencing have led to the identification of somatic mutations in isocitrate dehydrogenase 1 (IDH1) in various malignancies. IDH1 R132H is the most common mutation of IDH1, which affects codon 132 and results in the conversion of amino acid residue arginine (R) to histidine (H). This study is designed to evaluate the association between the expression of IDH1 R132H and clinicopathological characteristics in laryngeal squamous cell carcinoma (LSCC).
Methods
The expression pattern and clinical significance of IDH1 R132H were investigated in tissue microarrays (TMAs) of 50 LSCC tumors as well as adjacent normal tissues using immunohistochemistry. Then the exons of the 12 tumor samples with negative/weak positive staining were sequenced by applying polymerase chain reaction (PCR).
Results
The results demonstrated that the cytoplasmic expression of IDH1 R132H was downregulated in tumor cells compared to adjacent normal tissues. A statistically significant association was found between a low level of cytoplasmic expression of IDH1 R132H protein and an increase in histological grade (
p
< 0.001), perineural invasion (
p
= 0.019), and lymph node involvement (
p
< 0.001). The exon4 sequencing results showed that only one sample was positive for IDH1 R132H mutation. IDH1 R132H expression was observed in 39 (78.0%) LSCC samples.
Conclusion
These findings indicate that low cytoplasmic expression of IDH1 R132H may have clinical significance in LSCC patients and is associated with more aggressive tumor behavior and progression of the disease, which can help improve potential treatment in patients with LSCC. Further investigations are needed to understand the biological function of IDH1 R132H and larger sample size to confirm our findings.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>36063222</pmid><doi>10.1007/s00432-022-04336-z</doi><tpages>15</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Amino acids Cancer Research Clinical significance Dehydrogenases Exons Hematology Histidine Immunohistochemistry Internal Medicine Isocitrate dehydrogenase Laryngeal cancer Laryngeal carcinoma Lymph nodes Malignancy Medicine Medicine & Public Health Mutation Oncology Patients Polymerase chain reaction Squamous cell carcinoma Statistical analysis Tumor cells Tumors |
| title | Low expression of isocitrate dehydrogenase 1 (IDH1) R132H is associated with advanced pathological features in laryngeal squamous cell carcinoma |
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