Post-staining Raman analysis of histological sections following decolorization

Hematoxylin and eosin (HE) staining of tissue sections is a powerful tool for observing changes in the tissue structure and is used as the most fundamental and vital technique in histology. However, xenobiotics such as polymers and inorganic or organic materials have low dyeability, making it diffic...

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Bibliographic Details
Published in:Analyst (London) 2022-10, Vol.147 (2), p.4473-4479
Main Authors: Akaji, Sakiko, Sagawa, Tomoya, Honda, Akiko, Miyasaka, Natsuko, Sadakane, Kaori, Ichinose, Takamichi, Takano, Hirohisa
Format: Article
Language:English
Subjects:
Decoloring
Fluorescence
Histology
Organic materials
Raman spectroscopy
Staining
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Summary:Hematoxylin and eosin (HE) staining of tissue sections is a powerful tool for observing changes in the tissue structure and is used as the most fundamental and vital technique in histology. However, xenobiotics such as polymers and inorganic or organic materials have low dyeability, making it difficult to observe the distribution of materials across tissues. Raman spectroscopy is an advantageous technique for identifying materials in tissues using spectroscopic fingerprints by laser irradiation without staining. In this study, we developed a combined method for morphological observation and Raman spectral acquisition on the identical tissue slide by employing a decolorization step to remove eosin-induced fluorescence in HE-stained samples. Our method eliminated the fluorescence background and allowed the identical-field pathological observation, enabling simultaneous identification of biological responses and materials in tissues. This study proposed to observe HE-stained tissue structure and Raman fingerprint mapping on the identical tissue slide after a decolorizing step.
ISSN:0003-2654
1364-5528
DOI:10.1039/d2an01138g