Biotic elicitor–induced changes in growth, antioxidative defense, and metabolites in an improved prickleless Solanum viarum

• Published in: Applied microbiology and biotechnology 2022-10, Vol.106 (19-20), p.6455-6469
• Main Authors: Prasad, Archana, Patel, Preeti, Niranjan, Abhishek, Mishra, Aradhana, Saxena, Gauri, Singh, Satya Shila, Chakrabarty, Debasis
• Format: Article
• Language: English
• Subjects: Accumulation; Alkaloids; Ascorbic acid; Bacillus (Bacteria); Bioactive compounds; Biochemical analysis; Biomass; Biomedical and Life Sciences; Biosynthesis; Biotechnological Products and Process Engineering; Biotechnology; Carotenoids; Chemical properties; Damage; Filtrate; Flavonoids; Fungi; Glycoalkaloids; Herbal medicine; Identification and classification; L-Ascorbate peroxidase; Life Sciences; Medicinal plants; Metabolites; Microbial Genetics and Genomics; Microbiology; Microorganisms; Peroxidase; Phenols; Plant metabolites; Raw materials; Reactive oxygen species; Solanaceae; Solanum viarum; Superoxide dismutase; Varieties
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-022-12159-8

Solanum viarum serves as a raw material for the steroidal drug industry due to its alkaloid and glycoalkaloid content. Elicitation is well-known for measuring the increase in the yield of bioactive compounds in in vitro cultures. The current study was performed for the accumulation of metabolites viz. solasodine, solanidine, and α-solanine in S. viarum culture using microbial-based elicitors added in 1%, 3%, 5%, and 7% on 25 th and 35 th day of culture period and harvested on 45 th and 50 th days of culture cycle. The treatment of 3% Trichoderma reesei and Bacillus tequilensis culture filtrate (CF) significantly increased biomass, alkaloids/glycoalkaloid content, and yield in S. viarum. T. reesei was found to be the best treatment for enhanced growth (GI = 11.65) and glycoalkaloid yield (2.54 mg DW plant −1 ) after the 50 th day of the culture cycle when added on the 25 th day. The abundance of gene transcripts involved in the biosynthesis of alkaloids/glycoalkaloids, revealed by quantitative real-time PCR expression analysis correlates with the accumulation of their respective metabolites in elicited plants. Biochemical analysis shows that elicited plants inhibited oxidative damage caused by reactive oxygen species by activating enzymes (superoxide dismutase and ascorbate peroxidase) as well as non-enzymatic antioxidant mechanisms (alkaloids, total phenols, total flavonoids, carotenoids, and proline). The findings of this study clearly demonstrate that the application of T. reesei and B. tequilensis CF at a specific dose and time significantly improve biomass as well as upregulates the metabolite biosynthetic pathway in an important medicinal plant- S. viarum . Key points • Biotic elicitors stimulated the alkaloids/glycoalkaloid content in S. viarum plant cultures. • T. reesei was found to be most efficient for enhancing the growth and alkaloids content. • Elicited plants activate ROS based-defense mechanism to overcome oxidative damage.