Disposable stainless steel working electrodes for sensitive and simultaneous detection of indole-3-acetic acid and salicylic acid in Arabidopsis thaliana leaves under biotic stresses

The detection of phytohormones in real time has attracted increasing attention because of their critical roles in regulating the development and signaling of plants, especially in defense against biotic stresses. Herein, stainless steel sheet electrodes modified with carbon cement were coupled with...

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Published in:Analytical and bioanalytical chemistry 2022-11, Vol.414 (26), p.7721-7730
Main Authors: Sun, Ling, Xu, Songzhi, Tang, Yihui, Zhou, Yuhang, Wang, Meng, Tian, Yiran, Li, Guangxi, Zhu, Xinyu, Bao, Ning, Sun, Lijun
Format: Article
Language:English
Subjects:
Acetic acid
Acids
Agricultural technology
Analysis
Analytical Chemistry
Arabidopsis thaliana
Biochemistry
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Electric properties
Electrochemical analysis
Electrochemistry
Electrodes
Food Science
Indole
Indoleacetic acid
Laboratory Medicine
Leaves
Metal sheets
Monitoring/Environmental Analysis
Plant hormones
Precision farming
Research Paper
Salicylic acid
Sheet-steel
Stainless steel
Stainless steels
Stresses
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Summary:The detection of phytohormones in real time has attracted increasing attention because of their critical roles in regulating the development and signaling of plants, especially in defense against biotic stresses. Herein, stainless steel sheet electrodes modified with carbon cement were coupled with paper-based analysis devices for direct and simultaneous detection of salicylic acid (SA) and indole-3-acetic acid (IAA) in plants. We demonstrated that the excellent conductivity of stainless steel sheet electrodes enabled us to simultaneously differentiate IAA and SA at a level of 10 nM. With our approach, the content of IAA and SA in Arabidopsis thaliana leaves infected or not infected with Pst DC3000 could be rapidly quantified at the same time. Our experimental results on differentiation of IAA and SA at different time points showed that there were antagonistic interactions between the IAA and SA after infection of Arabidopsis leaves with Pst DC3000. By offering a cost-effective approach for rapid and sensitive detection of IAA and SA, this study suggests that electrochemical detection can be used in the study and development of precision agriculture technology. Graphical abstract
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-022-04303-0