Senegenin Rescues PC12 Cells with Oxidative Damage Through Inhibition of Ferroptosis

Oxidative stress is one of the pathological mechanisms of Alzheimer’s disease (AD), and ferroptosis has been determined to be involved in neurodegenerative diseases such as AD. Senegenin (Sen) prevents oxidative damage in nerve cells via a mechanism that may be highly related to ferroptosis. However...

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Bibliographic Details
Published in:Molecular neurobiology 2022-11, Vol.59 (11), p.6983-6992
Main Authors: Zhang, Heping, Zhou, Wei, Li, Jianling, Qiu, Zhaohui, Wang, Xiaotong, Xu, Hui, Wang, Huadong, Lu, Daxiang, Qi, Renbin
Format: Article
Language:English
Subjects:
Alzheimer's disease
Biomedical and Life Sciences
Biomedicine
Cell Biology
Cytotoxicity
Depolarization
Ferroptosis
Mitochondria
Neurobiology
Neurodegenerative diseases
Neurology
Neuroprotection
Neurosciences
Oxidative stress
Pheochromocytoma cells
Proteins
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Summary:Oxidative stress is one of the pathological mechanisms of Alzheimer’s disease (AD), and ferroptosis has been determined to be involved in neurodegenerative diseases such as AD. Senegenin (Sen) prevents oxidative damage in nerve cells via a mechanism that may be highly related to ferroptosis. However, the mechanism of ferroptosis pathway involvement in AD is unclear. In this study, we established a model of PC12 cytotoxic injury induced by Aβ 25–35 , and we detected the level of oxidative damage, MMP, and ferroptosis-related protein expression. The results showed that, compared with control group, the level of ROS increased, GPX activities decreased, and MDA levels increased in Aβ 25–35 group. Aβ 25–35 could induce mitochondrial depolarization in PC12 cells and Fer-1 could not reverse this damage. WB revealed that Aβ 25–35 group had increased ACSL4 and PEBP1 proteins, and decreased GPX4 protein. After adding Sen in the model, the level of oxidative damage was reduced, and mitochondrial depolarization was reversed compared with Aβ 25–35 group. WB suggested that the expression of ACSL4 and PEBP1 proteins decreased, and the expression of GPX4 protein increased by Sen treatment. In conclusion, we found that Sen exhibits strong neuroprotective activity against Aβ 25–35 induced oxidative damage and lipid metabolic associated with ferroptosis. Inhibiting nerve cell ferroptosis might facilitate the future development of strategies to AD.
ISSN:0893-7648
1559-1182
DOI:10.1007/s12035-022-03014-y