Amomum longiligulare polysaccharide 1- PLGA nanoparticle promotes the immune activities of T lymphocytes and dendritic cells

• Published in: International immunopharmacology 2022-11, Vol.112, p.109204-109204, Article 109204
• Main Authors: Wu, Haowen, Chen, Huricha, Liu, Jiaguo, Xing, Zengyang, Ni, Jiahao, Teng, Ling, Chen, Yun
• Format: Article
• Language: English
• Subjects: Amomum longiligulare polysaccharide 1; Immunomodulatory activity; Poly (lactic-co-glycolic acid)
• ISSN: 1567-5769; 1878-1705
• DOI: 10.1016/j.intimp.2022.109204

•Amomum longiligulare polysaccharide 1 (ALP1) was prepared to nanoparticle (ALPP).•ALP1 and ALPP promoted the proliferation of T lymphocytes and Th1 immune response.•ALP1 and ALPP promoted the proliferation and maturation of chBM-DCs.•ALPP was much more effectively swallowed by chBM-DCs than ALP1.•The immunomodulatory activity of ALPP was higher than that of ALP1. Amomum longiligulare polysaccharide 1 (ALP1) was extracted from Amomum longiligulare T.L. Wu fruits and the poly (lactic-co-glycolic acid) nanoparticle enveloping ALP1 (ALPP) showed a good promoting effect on the activation of macrophages in our previous study. To further understand the immunomodulatory property of ALPP, the effect of ALPP on T lymphocytes and dendritic cells was investigated in the present study. The proliferation rates of chicken T lymphocytes and chicken bone marrow dendritic cells (chBM-DCs) that were treated with ALP1 or ALPP were determined by using MTT method. Meanwhile, the relative mRNA levels of cytokines from T lymphocytes and surface molecules of chBM-DCs were determined by using qRT-PCR method. In addition, the drug uptake capacity of chBM-DCs was also tested. As a result, the promoting effect on the proliferation of T lymphocytes and the Th1-type immune response of ALPP was better than that of ALP1. In addition, ALPP was much more effectively swallowed by chBM-DCs so that its promoting effect on the proliferation and maturation of chBM-DCs was higher than that of ALP1. To conclude, ALPP had a stronger immunomodulatory activity than ALP1, and showed the potential to become a new type of immune booster.