The potential therapeutic effect of klotho on cell viability in human colorectal adenocarcinoma HT-29 cells

Klotho is an anti-aging, anti-inflammator, and anti-oxidative protein and has been shown to important role in tumorigenesis, proliferation, survival, autophagy, and resistance to tumor suppressor effects in several types of human cancers. In this study, we aimed to investigate possible anti-tümör an...

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Published in:Medical oncology (Northwood, London, England) London, England), 2022-09, Vol.39 (12), p.191-191, Article 191
Main Authors: Sariboyaci, Ayla Eker, Uysal, Onur, Soykan, Merve Nur, Gunes, Sibel
Format: Article
Language:English
Subjects:
Aging
Apoptosis
Cancer
Colorectal cancer
Cytotoxicity
Flow cytometry
Gene expression
Health sciences
Hematology
Internal Medicine
Kinases
Medicine
Medicine & Public Health
Morphology
Oncology
Original Paper
Pathology
Proteins
Statistical analysis
Stem cells
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Summary:Klotho is an anti-aging, anti-inflammator, and anti-oxidative protein and has been shown to important role in tumorigenesis, proliferation, survival, autophagy, and resistance to tumor suppressor effects in several types of human cancers. In this study, we aimed to investigate possible anti-tümör and apoptotic effects of exogen klotho in human colorectal adenocarcinoma cells (HT-29) and healthy colon cells (CCD 841 CoN). The WST-8 test was used to determine the half-maximum inhibitory concentration (IC50) of the klotho protein. AO-PI fluorescent staining techniques and Annexin V-PI flow cytometry was utilized to observe and detect the apoptosis of cancer cells induced by klotho. Our results demonstrated that klotho had a cytotoxic effect against colorectal adenocarcinoma cells in a dose-dependent manner. Our Annexin V-PI flow cytometric and AO-PI fluorescent analyses showed that klotho induced quantitative and morphological changes that indicate apoptotic induction in the human colorectal adenocarcinoma. This study results proved for the first time that klotho may be an effective potential therapeutic agent that may be used in adjuvant therapy in human colorectal adenocarcinoma it does not affect selectively healthy colon cells and but leading cancer cells to apoptosis.
ISSN:1559-131X
1357-0560
1559-131X
DOI:10.1007/s12032-022-01793-x