Preparation of PEI‐modified nanoparticles by dopamine self‐polymerization for efficient DNA delivery

Achieving efficient and safe gene delivery is of great significance to promote the development of gene therapy. In this work, a polydopamine (PDA) layer was coated on the surface of Fe3O4 nanoparticles (NPs) by dopamine (DA) self‐polymerization, and then magnetic Fe3O4 NPs were prepared by the Micha...

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Bibliographic Details
Published in:Biotechnology and applied biochemistry 2023-04, Vol.70 (2), p.824-834
Main Authors: Liu, Liang, Yang, Zhaojun, Liu, Chaobing, Wang, Mengying, Chen, Xin
Format: Article
Language:English
Subjects:
Amino groups
Atomic force microscopy
Deoxyribonucleic acid
DNA
DNA - genetics
Dopamine
Electrophoresis
Fe3O4 nanoparticle
Flow cytometry
Fluorescence
Fluorescence microscopy
Fourier transforms
Gel electrophoresis
gene delivery
Gene therapy
Gene transfer
Iron oxides
Microscopy
Nanoparticles
Nuclease
Polyethyleneimine
Polymerization
Scanning electron microscopy
self‐polymerization
Transfection
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Summary:Achieving efficient and safe gene delivery is of great significance to promote the development of gene therapy. In this work, a polydopamine (PDA) layer was coated on the surface of Fe3O4 nanoparticles (NPs) by dopamine (DA) self‐polymerization, and then magnetic Fe3O4 NPs were prepared by the Michael addition between amino groups in polyethyleneimine (PEI) and PDA. The prepared Fe3O4 NPs (named Fe3O4@PDA@PEI) were characterized by Fourier transform infrared (FTIR), atomic force microscopy (AFM), and scanning electron microscopy (SEM). As an efficient and safe gene carrier, the potential of Fe3O4@PDA@PEI was evaluated by agarose gel electrophoresis, 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide assay, fluorescence microscopy, and flow cytometry. The results show that the Fe3O4@PDA@PEI NPs are stable hydrophilic NPs with a particle size of 50–150 nm. It can efficiently condense DNA at low N/P ratios and protect it from nuclease degradation. In addition, the Fe3O4@PDA@PEI NPs have higher safety than PEI. Further, the Fe3O4@PDA@PEI/DNA polyplexes could be effectively absorbed by cells and successfully transfected and exhibit higher cellular uptake and gene transfection efficiency than PEI/DNA polyplexes. The findings indicate that the Fe3O4@PDA@PEI NPs have the potential to be developed into a novel gene vector.  
ISSN:0885-4513
1470-8744
DOI:10.1002/bab.2402