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Intrinsic chlorine resistance of bacteria modulated by glutaminyl-tRNA biosynthesis in drinking water supply systems

The existence of chlorine-resistant bacteria (CRB) in drinking water supply systems (DWSSs) results in significant challenges to the biological security of drinking water. However, little is known about the intrinsic chlorine-resistant molecular metabolic mechanism of bacteria in DWSSs. This researc...

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Published in:Chemosphere (Oxford) 2022-12, Vol.308, p.136322-136322, Article 136322
Main Authors: Miao, Xiaocao, Han, Xue, Liu, Chenxu, Bai, Xiaohui
Format: Article
Language:English
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Summary:The existence of chlorine-resistant bacteria (CRB) in drinking water supply systems (DWSSs) results in significant challenges to the biological security of drinking water. However, little is known about the intrinsic chlorine-resistant molecular metabolic mechanism of bacteria in DWSSs. This research explored the microbial interactions and the key metabolic pathways that modulate the chlorine resistance of bacteria in full-scale chloraminated DWSSs. The dominant CRB, including Bdellovibrio, Bradyrhizobium, Peredibacter, Sphingomonas, and Hydrogenophaga, strongly interacted with each other to maintain basic metabolism. A total of 4.21% of the bacterial metabolic pathways were key and specific to chlorine-resistant bacteria. Glutaminyl-tRNA biosynthesis was the dominant metabolic pathway of CRB in the target DWSSs. After chloramine disinfection, the relative abundance of glutamate-tRNA ligase (GlnRS) and the related orthologous genes increased by 10.11% and 14.58%, respectively. The inactivation rate of the GlnRS overexpression strain (81.40%) was lower than that of the wild-type strain (90.11%) after exposure to chloramine. Meanwhile, the growth rate of the GlnRS overexpression strain was higher than that of the wild-type strain. Glutaminyl-tRNA biosynthesis can enhance chlorine resistance in DWSSs. [Display omitted] •The dominant CRB interacted strongly to maintain basic metabolism.•Glutaminyl-tRNA biosynthesis was the key metabolic pathway of CRB.•The GlnRS overexpression strain showed a lower inactivation rate after chloramination.•The chlorine resistance of bacteria can be modulated by GlnRS in DWSSs.
ISSN:0045-6535
1879-1298
DOI:10.1016/j.chemosphere.2022.136322