The effect of minocycline on beta-amyloid-induced memory and learning deficit in male rats: A behavioral, biochemical, and histological study

• Published in: Journal of chemical neuroanatomy 2022-11, Vol.125, p.102158-102158, Article 102158
• Main Authors: Gholami Mahmoudian, Zahra, komaki, Alireza, Rashidi, Iraj, Amiri, Iraj, Ghanbari, Ali
• Format: Article
• Language: English
• Subjects: Alzheimer’s disease; Apoptosis; Hippocampus; Minocycline; Rats
• ISSN: 0891-0618; 1873-6300
• DOI: 10.1016/j.jchemneu.2022.102158

Minocycline hydrochloride is a semi-synthetic, second-generation tetracycline with neuroprotective, neurorestorative, anti-amyloidogenic, anti-inflammatory, antioxidant, and anti-apoptotic properties. The present study was designed to investigate the potential protective effects of minocycline against beta-amyloid (Aβ)-induced Alzheimer’s disease (AD), recognition memory decline, and the possible involved anti-apoptotic mechanisms. The rats were treated with minocycline (50 and 100 mg/kg/day; P.O.) after AD induction for 30 days. Behavioral functions were assessed by employing standard behavioral tests, including novel object recognition (NOR) and passive avoidance learning (PAL) tasks. Then, total antioxidant capacity (TAC) and total oxidant status (TOS) were measured in blood serum using ELISA kits. Apoptosis and the number of Aβ plaques were examined by the TUNEL and Congo red staining, respectively. Treatment of Aβ rats with minocycline improved memory deficit in the PAL task and a decline in recognition memory in the NOR test. Minocycline at 50 and 100 mg/kg significantly reduced the TOS levels and increased the TAC levels (P