Raman spectroscopy for the determination of forensically important bio-fluids

Body fluid (blood, sperm, saliva, and vaginal secretions) trace evidence acquired from crime scenes is increasingly being used by scientists for phenotypic profiling and DNA analysis to identify the source of the body fluid. Chemical tests, which are costly, time-consuming, and require pre-sample pr...

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Bibliographic Details
Published in:Forensic science international 2022-11, Vol.340, p.111441-111441, Article 111441
Main Authors: George, Noble, Singh, Harjeet, Jotaniya, Rajashree, Pandya, Shivani R.
Format: Article
Language:English
Subjects:
Backscattering
Biological properties
Biological samples
Body fluids
Chemical tests
Crime
Crime scenes
Criminal investigations
DNA fingerprinting
DNA methylation
Evidence
Forensic chemistry
Forensic sciences
Humidity
Laboratories
Raman spectra
Raman spectroscopy
Saliva
Sample preparation
Scientists
Secretions
Spectroscopy
Spectrum analysis
Sperm
Stains & staining
Urine
Vagina
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Summary:Body fluid (blood, sperm, saliva, and vaginal secretions) trace evidence acquired from crime scenes is increasingly being used by scientists for phenotypic profiling and DNA analysis to identify the source of the body fluid. Chemical tests, which are costly, time-consuming, and require pre-sample preparation, were used heavily by scientists in the past. Furthermore, each test is limited to a single sample. Raman spectroscopy (RS) is a universal technique for analyzing various substances as it measures backscatter. The Portable RS is now commonly utilized at crime scenes. It is quick and requires no sample preparation. The biological samples can also be used for prospective analysis because the procedure is non-destructive. Because of its extremely selective nature, it can also examine heterogeneous samples and separate each ingredient. Different bodily fluids have distinct Raman spectra. A stain that contains multiple body fluids could produce a superposition of all the peaks that can be mathematically resolved into separate fundamental spectra. In this review, we focused on the use of RS in the identification and characterisation of bodily fluids at crime scenes. Also, this review emphasized the merits of choosing RS over other conventional methods.
ISSN:0379-0738
1872-6283
DOI:10.1016/j.forsciint.2022.111441