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Chlorogenic acid improves functional potential of follicles in mouse whole ovarian tissues in vitro
Background Chlorogenic acid (CGA) is one of the well-known polyphenol compounds possessing several important biological and therapeutic functions. In order to optimize a culture system to achieve complete development of follicles, we focused on the effects of CGA supplementation during in vitro cult...
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| Published in: | Molecular biology reports 2022-11, Vol.49 (11), p.10327-10338 |
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| creator | Abedpour, Neda Javanmard, Masoumeh Zirak Karimipour, Mojtaba Farjah, Gholam Hossein |
| description | Background
Chlorogenic acid
(CGA) is one of the well-known polyphenol compounds possessing several important biological and therapeutic functions. In order to optimize a culture system to achieve complete development of follicles, we focused on the effects of CGA supplementation during
in vitro
culture (IVC) on follicular development, oxidative stress, antioxidant capacity, developmental gene expression, and functional potential in cultured mouse ovarian tissue.
Methods and results
The collected whole murine ovaries were randomly divided into four groups: (1) non-cultured group (control 1) with 7-day-old mouse ovaries, (2) non-cultured group (control 2) with 14-day-old mouse ovaries, (3) cultured group (experimental 1) with the culture plates containing only the basic culture medium, (4) cultured group (experimental 2) with the culture plates containing basic culture medium + CGA (50, 100 and 200 µmol/L CGA). Afterward, histological evaluation, biochemical analyses, the expression assessment of genes related to follicular development and apoptosis as well as the analysis of 17-β-estradiol were performed. The results showed that supplementation of ovarian tissue with the basic culture media using CGA (100 µmol/l) significantly increased the survival, developmental and functional potential of follicles in whole mouse ovarian tissues after 7 days of culture. Furthermore, CGA (100 µmol/L) attenuated oxidative damage and enhanced the concentration of antioxidant capacity along with developmental gene expression.
Conclusion
It seems that supplementation of ovarian tissue with culture media using CGA could optimize follicular growth and development in the culture system. |
| doi_str_mv | 10.1007/s11033-022-07793-4 |
| format | article |
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Chlorogenic acid
(CGA) is one of the well-known polyphenol compounds possessing several important biological and therapeutic functions. In order to optimize a culture system to achieve complete development of follicles, we focused on the effects of CGA supplementation during
in vitro
culture (IVC) on follicular development, oxidative stress, antioxidant capacity, developmental gene expression, and functional potential in cultured mouse ovarian tissue.
Methods and results
The collected whole murine ovaries were randomly divided into four groups: (1) non-cultured group (control 1) with 7-day-old mouse ovaries, (2) non-cultured group (control 2) with 14-day-old mouse ovaries, (3) cultured group (experimental 1) with the culture plates containing only the basic culture medium, (4) cultured group (experimental 2) with the culture plates containing basic culture medium + CGA (50, 100 and 200 µmol/L CGA). Afterward, histological evaluation, biochemical analyses, the expression assessment of genes related to follicular development and apoptosis as well as the analysis of 17-β-estradiol were performed. The results showed that supplementation of ovarian tissue with the basic culture media using CGA (100 µmol/l) significantly increased the survival, developmental and functional potential of follicles in whole mouse ovarian tissues after 7 days of culture. Furthermore, CGA (100 µmol/L) attenuated oxidative damage and enhanced the concentration of antioxidant capacity along with developmental gene expression.
Conclusion
It seems that supplementation of ovarian tissue with culture media using CGA could optimize follicular growth and development in the culture system.</description><identifier>ISSN: 0301-4851</identifier><identifier>EISSN: 1573-4978</identifier><identifier>DOI: 10.1007/s11033-022-07793-4</identifier><identifier>PMID: 36097112</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>17β-Estradiol ; Animal Anatomy ; Animal Biochemistry ; Animals ; antioxidant activity ; Antioxidants ; Antioxidants - metabolism ; Antioxidants - pharmacology ; Apoptosis ; Biomedical and Life Sciences ; Chlorogenic acid ; Chlorogenic Acid - metabolism ; Chlorogenic Acid - pharmacology ; Culture media ; Culture Media - metabolism ; Culture Media - pharmacology ; Female ; Follicles ; follicular development ; Gene expression ; growth and development ; Histology ; Life Sciences ; Mice ; Morphology ; Original Article ; Ovarian Follicle ; Ovaries ; Ovary ; Oxidative stress ; polyphenols ; Supplements ; therapeutics ; Tissue culture</subject><ispartof>Molecular biology reports, 2022-11, Vol.49 (11), p.10327-10338</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2022. corrected publication 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2022. The Author(s), under exclusive licence to Springer Nature B.V.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c452t-a39f20fe2c6af733f20028f4c52371caeea36c79933679dfde465ba81890a6643</citedby><cites>FETCH-LOGICAL-c452t-a39f20fe2c6af733f20028f4c52371caeea36c79933679dfde465ba81890a6643</cites><orcidid>0000-0002-7880-3263</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36097112$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Abedpour, Neda</creatorcontrib><creatorcontrib>Javanmard, Masoumeh Zirak</creatorcontrib><creatorcontrib>Karimipour, Mojtaba</creatorcontrib><creatorcontrib>Farjah, Gholam Hossein</creatorcontrib><title>Chlorogenic acid improves functional potential of follicles in mouse whole ovarian tissues in vitro</title><title>Molecular biology reports</title><addtitle>Mol Biol Rep</addtitle><addtitle>Mol Biol Rep</addtitle><description>Background
Chlorogenic acid
(CGA) is one of the well-known polyphenol compounds possessing several important biological and therapeutic functions. In order to optimize a culture system to achieve complete development of follicles, we focused on the effects of CGA supplementation during
in vitro
culture (IVC) on follicular development, oxidative stress, antioxidant capacity, developmental gene expression, and functional potential in cultured mouse ovarian tissue.
Methods and results
The collected whole murine ovaries were randomly divided into four groups: (1) non-cultured group (control 1) with 7-day-old mouse ovaries, (2) non-cultured group (control 2) with 14-day-old mouse ovaries, (3) cultured group (experimental 1) with the culture plates containing only the basic culture medium, (4) cultured group (experimental 2) with the culture plates containing basic culture medium + CGA (50, 100 and 200 µmol/L CGA). Afterward, histological evaluation, biochemical analyses, the expression assessment of genes related to follicular development and apoptosis as well as the analysis of 17-β-estradiol were performed. The results showed that supplementation of ovarian tissue with the basic culture media using CGA (100 µmol/l) significantly increased the survival, developmental and functional potential of follicles in whole mouse ovarian tissues after 7 days of culture. Furthermore, CGA (100 µmol/L) attenuated oxidative damage and enhanced the concentration of antioxidant capacity along with developmental gene expression.
Conclusion
It seems that supplementation of ovarian tissue with culture media using CGA could optimize follicular growth and development in the culture system.</description><subject>17β-Estradiol</subject><subject>Animal Anatomy</subject><subject>Animal Biochemistry</subject><subject>Animals</subject><subject>antioxidant activity</subject><subject>Antioxidants</subject><subject>Antioxidants - metabolism</subject><subject>Antioxidants - pharmacology</subject><subject>Apoptosis</subject><subject>Biomedical and Life Sciences</subject><subject>Chlorogenic acid</subject><subject>Chlorogenic Acid - metabolism</subject><subject>Chlorogenic Acid - pharmacology</subject><subject>Culture media</subject><subject>Culture Media - metabolism</subject><subject>Culture Media - pharmacology</subject><subject>Female</subject><subject>Follicles</subject><subject>follicular development</subject><subject>Gene expression</subject><subject>growth and development</subject><subject>Histology</subject><subject>Life Sciences</subject><subject>Mice</subject><subject>Morphology</subject><subject>Original Article</subject><subject>Ovarian Follicle</subject><subject>Ovaries</subject><subject>Ovary</subject><subject>Oxidative stress</subject><subject>polyphenols</subject><subject>Supplements</subject><subject>therapeutics</subject><subject>Tissue culture</subject><issn>0301-4851</issn><issn>1573-4978</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNqFkUtvGyEUhVGVqHHc_oEuIqRsupn28pgBlpWVJpUiddOuEcFgEzGDAzOO-u-DM3lIXSQrLrof5x7uQegLgW8EQHwvhABjDVDagBCKNfwDWpBW1EIJeYQWwIA0XLbkBJ2WcgsAnIj2IzphHShBCF0gu9rGlNPGDcFiY8Mah36X094V7KfBjiENJuJdGt0whlolj32KMdhYiTDgPk3F4fttig6nvcnBDHgMpUxzex_GnD6hY29icZ-fziX6-_Piz-qquf59-Wv147qxvKVjY5jyFLyjtjNeMFYvQKXntqVMEGucM6yzQinGOqHWfu14194YSaQC03WcLdHXWbd-4K4aGHUfinUxmsFVm5pKrqRiVML7qCAcOoA6a4nO_0Nv05TrVg5UXbCQqj0I0pmyOZWSnde7HHqT_2kC-pCWntPSNS39mJY-GD57kp5uerd-efIcTwXYDJTaGjYuv85-Q_YB_fyfmQ</recordid><startdate>20221101</startdate><enddate>20221101</enddate><creator>Abedpour, Neda</creator><creator>Javanmard, Masoumeh Zirak</creator><creator>Karimipour, Mojtaba</creator><creator>Farjah, Gholam Hossein</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0002-7880-3263</orcidid></search><sort><creationdate>20221101</creationdate><title>Chlorogenic acid improves functional potential of follicles in mouse whole ovarian tissues in vitro</title><author>Abedpour, Neda ; Javanmard, Masoumeh Zirak ; Karimipour, Mojtaba ; Farjah, Gholam Hossein</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-a39f20fe2c6af733f20028f4c52371caeea36c79933679dfde465ba81890a6643</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>17β-Estradiol</topic><topic>Animal Anatomy</topic><topic>Animal Biochemistry</topic><topic>Animals</topic><topic>antioxidant activity</topic><topic>Antioxidants</topic><topic>Antioxidants - metabolism</topic><topic>Antioxidants - pharmacology</topic><topic>Apoptosis</topic><topic>Biomedical and Life Sciences</topic><topic>Chlorogenic acid</topic><topic>Chlorogenic Acid - metabolism</topic><topic>Chlorogenic Acid - pharmacology</topic><topic>Culture media</topic><topic>Culture Media - metabolism</topic><topic>Culture Media - pharmacology</topic><topic>Female</topic><topic>Follicles</topic><topic>follicular development</topic><topic>Gene expression</topic><topic>growth and development</topic><topic>Histology</topic><topic>Life Sciences</topic><topic>Mice</topic><topic>Morphology</topic><topic>Original Article</topic><topic>Ovarian Follicle</topic><topic>Ovaries</topic><topic>Ovary</topic><topic>Oxidative stress</topic><topic>polyphenols</topic><topic>Supplements</topic><topic>therapeutics</topic><topic>Tissue culture</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Abedpour, Neda</creatorcontrib><creatorcontrib>Javanmard, Masoumeh Zirak</creatorcontrib><creatorcontrib>Karimipour, Mojtaba</creatorcontrib><creatorcontrib>Farjah, Gholam Hossein</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Science Journals</collection><collection>ProQuest Biological Science Journals</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Molecular biology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Abedpour, Neda</au><au>Javanmard, Masoumeh Zirak</au><au>Karimipour, Mojtaba</au><au>Farjah, Gholam Hossein</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chlorogenic acid improves functional potential of follicles in mouse whole ovarian tissues in vitro</atitle><jtitle>Molecular biology reports</jtitle><stitle>Mol Biol Rep</stitle><addtitle>Mol Biol Rep</addtitle><date>2022-11-01</date><risdate>2022</risdate><volume>49</volume><issue>11</issue><spage>10327</spage><epage>10338</epage><pages>10327-10338</pages><issn>0301-4851</issn><eissn>1573-4978</eissn><abstract>Background
Chlorogenic acid
(CGA) is one of the well-known polyphenol compounds possessing several important biological and therapeutic functions. In order to optimize a culture system to achieve complete development of follicles, we focused on the effects of CGA supplementation during
in vitro
culture (IVC) on follicular development, oxidative stress, antioxidant capacity, developmental gene expression, and functional potential in cultured mouse ovarian tissue.
Methods and results
The collected whole murine ovaries were randomly divided into four groups: (1) non-cultured group (control 1) with 7-day-old mouse ovaries, (2) non-cultured group (control 2) with 14-day-old mouse ovaries, (3) cultured group (experimental 1) with the culture plates containing only the basic culture medium, (4) cultured group (experimental 2) with the culture plates containing basic culture medium + CGA (50, 100 and 200 µmol/L CGA). Afterward, histological evaluation, biochemical analyses, the expression assessment of genes related to follicular development and apoptosis as well as the analysis of 17-β-estradiol were performed. The results showed that supplementation of ovarian tissue with the basic culture media using CGA (100 µmol/l) significantly increased the survival, developmental and functional potential of follicles in whole mouse ovarian tissues after 7 days of culture. Furthermore, CGA (100 µmol/L) attenuated oxidative damage and enhanced the concentration of antioxidant capacity along with developmental gene expression.
Conclusion
It seems that supplementation of ovarian tissue with culture media using CGA could optimize follicular growth and development in the culture system.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>36097112</pmid><doi>10.1007/s11033-022-07793-4</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0002-7880-3263</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | 17β-Estradiol Animal Anatomy Animal Biochemistry Animals antioxidant activity Antioxidants Antioxidants - metabolism Antioxidants - pharmacology Apoptosis Biomedical and Life Sciences Chlorogenic acid Chlorogenic Acid - metabolism Chlorogenic Acid - pharmacology Culture media Culture Media - metabolism Culture Media - pharmacology Female Follicles follicular development Gene expression growth and development Histology Life Sciences Mice Morphology Original Article Ovarian Follicle Ovaries Ovary Oxidative stress polyphenols Supplements therapeutics Tissue culture |
| title | Chlorogenic acid improves functional potential of follicles in mouse whole ovarian tissues in vitro |
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