Effects of cysteamine supplementation on cryopreserved buffalo bull semen quality parameters

This work aimed to determine the effect of cysteamine (25, 50, 100 and 200 μM) incorporated during dilution on frozen thawed buffalo semen quality. Semen was collected twice weekly for 7 consecutive weeks from three Egyptian buffalo bulls using an artificial vagina. Semen samples were pooled and ext...

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Bibliographic Details
Published in:Theriogenology 2022-10, Vol.192, p.141-149
Main Authors: Reda Elkhawagah, Ahmed, Martino, Nicola Antonio, Moustafa Kandiel, Mohamed Mahmoud, Ghoneimy Mahmoud, Karima, Ricci, Alessandro, Vincenti, Leila
Format: Article
Language:English
Subjects:
Antioxidant capacity
Buffalo spermatozoa
Comet assay
Cryopreservation
Cysteamine
IVF
Transaminases
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Summary:This work aimed to determine the effect of cysteamine (25, 50, 100 and 200 μM) incorporated during dilution on frozen thawed buffalo semen quality. Semen was collected twice weekly for 7 consecutive weeks from three Egyptian buffalo bulls using an artificial vagina. Semen samples were pooled and extended with a Tris-based extender, cooled, equilibrated and finally frozen in liquid nitrogen. The diluted semen was evaluated for motility, viability, morphology, plasma membrane and DNA integrity, in addition to oxidative stress and in vitro fertilizing capability. The post thaw motility and velocity parameters noticeably increased with different concentrations of cysteamine (mainly 100 μM) during different incubation periods. The post thaw sperm viability and normality significantly (p 
ISSN:0093-691X
1879-3231
DOI:10.1016/j.theriogenology.2022.08.022