Maize cytosolic invertase INVAN6 ensures faithful meiotic progression under heat stress

Summary Faithful meiotic progression ensures the generation of viable gametes. Studies suggested the male meiosis of plants is sensitive to ambient temperature, but the underlying molecular mechanisms remain elusive. Here, we characterized a maize (Zea mays ssp. mays L.) dominant male sterile mutant...

Full description

Saved in:
Bibliographic Details
Published in:The New phytologist 2022-12, Vol.236 (6), p.2172-2188
Main Authors: Huang, Wei, Li, Yunfei, Du, Yan, Pan, Lingling, Huang, Yumin, Liu, Hongbing, Zhao, Yue, Shi, Yunlu, Ruan, Yong‐Ling, Dong, Zhaobin, Jin, Weiwei
Format: Article
Language:English
Subjects:
Ambient temperature
Corn
cytosolic invertase
Defects
Gametes
Heat
Heat stress
Heat tolerance
Homeostasis
Invertase
male sterility
Males
Meiosis
Metabolism
Molecular modelling
Mutants
Pachytene
Pollen
Proteins
Saccharides
Sucrose
Sugar
Transcriptomes
Zea mays
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Summary Faithful meiotic progression ensures the generation of viable gametes. Studies suggested the male meiosis of plants is sensitive to ambient temperature, but the underlying molecular mechanisms remain elusive. Here, we characterized a maize (Zea mays ssp. mays L.) dominant male sterile mutant Mei025, in which the meiotic process of pollen mother cells (PMCs) was arrested after pachytene. An Asp‐to‐Asn replacement at position 276 of INVERTASE ALKALINE NEUTRAL 6 (INVAN6), a cytosolic invertase (CIN) that predominantly exists in PMCs and specifically hydrolyses sucrose, was revealed to cause meiotic defects in Mei025. INVAN6 interacts with itself as well as with four other CINs and seven 14‐3‐3 proteins. Although INVAN6Mei025, the variant of INVAN6 found in Mei025, lacks hydrolytic activity entirely, its presence is deleterious to male meiosis, possibly in a dominant negative repression manner through interacting with its partner proteins. Notably, heat stress aggravated meiotic defects in invan6 null mutant. Further transcriptome data suggest INVAN6 has a fundamental role for sugar homeostasis and stress tolerance of male meiocytes. In summary, this work uncovered the function of maize CIN in male meiosis and revealed the role of CIN‐mediated sugar metabolism and signalling in meiotic progression under heat stress.
ISSN:0028-646X
1469-8137
DOI:10.1111/nph.18490