Orthotopic and heterotopic triple negative breast cancer preclinical murine models: A tumor microenvironment comparative

Breast cancer is the most frequent type of cancer worldwide and triple negative breast cancer is a particularly aggressive subtype. Novel therapies for the treatment of cancer patients focus on the remodeling of the tumor microenvironment (TME). Orthotopic and heterotopic syngeneic mice are the most...

Full description

Saved in:
Bibliographic Details
Published in:Research in veterinary science 2022-12, Vol.152, p.364-371
Main Authors: Santana-Krímskaya, Silvia Elena, Kawas, Jorge R., Zarate-Triviño, Diana Ginette, Ramos-Zayas, Yareellys, Rodríguez-Padilla, Cristina, Franco-Molina, Moisés Armides
Format: Article
Language:English
Subjects:
Air-pouch
Animal models
Antibodies
Binding sites
Breast cancer
Chemotherapy
Design of experiments
Experimental design
Extracellular matrix
Foxp3 protein
Heterotopic
Immune system
Laboratory animals
Mammary fatpad
Metastases
Orthotopic
Tumor microenvironment
Tumors
Veterinary medicine
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Breast cancer is the most frequent type of cancer worldwide and triple negative breast cancer is a particularly aggressive subtype. Novel therapies for the treatment of cancer patients focus on the remodeling of the tumor microenvironment (TME). Orthotopic and heterotopic syngeneic mice are the most common model used to study the TME in preclinical research. Despite this, there are no published studies that address the differences between orthotopic and heterotopic murine breast cancer models at the TME level. In this report we compared proliferation, immune cell infiltrates, extracellular matrix, vascular density, and response to chemotherapy between the mammary fat pad orthotopic model, and the air pouch heterotopic model. Our study shows that the orthotopic tumors form more metastasis, however, the heterotopic tumors grow larger, have a higher FOXP3 cell infiltrate, and resemble more accurately the breast cancer TME. Our findings show that both models are very similar, there are however some differences that should be considered in the experimental design of preclinical studies. •Breast cancer progression and resistance to therapy is supported by the tumor microenvironment (TME).•Orthotopic and heterotopic syngeneic mice are the most common model used to study the TME.•Similarities and differences between both models in the TME must be settled to choose the most appropriate for each study.
ISSN:0034-5288
1532-2661
DOI:10.1016/j.rvsc.2022.08.026