Enhanced tolerance of Cupriavidus necator NCIMB 11599 to lignocellulosic derived inhibitors by inserting NAD salvage pathway genes

Polyhydroxybutyrate (PHB) is a bio-based, biodegradable and biocompatible plastic that has the potential to replace petroleum-based plastics. Lignocellulosic biomass is a promising feedstock for industrial fermentation to produce bioproducts such as polyhydroxybutyrate (PHB). However, the pretreatme...

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Published in:Bioprocess and biosystems engineering 2022-10, Vol.45 (10), p.1719-1729
Main Authors: Lee, Sun Mi, Cho, Do-Hyun, Jung, Hee Ju, Kim, Byungchan, Kim, Su Hyun, Bhatia, Shashi Kant, Gurav, Ranjit, Jeon, Jong-Min, Yoon, Jeong-Jun, Park, Jeong-Hoon, Park, Jung-Ho, Kim, Yun-Gon, Yang, Yung-Hun
Format: Article
Language:English
Subjects:
Acetic acid
Biocompatibility
Biodegradability
Biodegradation
Biomass
Biotechnology
Chemistry
Chemistry and Materials Science
Cupriavidus necator
Environmental Engineering/Biotechnology
Fermentation
Food Science
Furfural
Genes
Genetic engineering
Hydrolysates
Industrial and Production Engineering
Industrial Chemistry/Chemical Engineering
Inhibitors
Lignocellulose
Microorganisms
NAD
Polyhydroxybutyrate
Polyhydroxybutyric acid
Research Paper
Toxicity
Vanillin
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Summary:Polyhydroxybutyrate (PHB) is a bio-based, biodegradable and biocompatible plastic that has the potential to replace petroleum-based plastics. Lignocellulosic biomass is a promising feedstock for industrial fermentation to produce bioproducts such as polyhydroxybutyrate (PHB). However, the pretreatment processes of lignocellulosic biomass lead to the generation of toxic byproducts, such as furfural, 5-HMF, vanillin, and acetate, which affect microbial growth and productivity. In this study, to reduce furfural toxicity during PHB production from lignocellulosic hydrolysates, we genetically engineered Cupriavidus necator NCIMB 11599, by inserting the nicotine amide salvage pathway genes pncB and nadE to increase the NAD(P)H pool. We found that the expression of pncB was the most effective in improving tolerance to inhibitors, cell growth, PHB production and sugar consumption rate. In addition, the engineered strain harboring pncB showed higher PHB production using lignocellulosic hydrolysates than the wild-type strain. Therefore, the application of NAD salvage pathway genes improves the tolerance of Cupriavidus necator to lignocellulosic-derived inhibitors and should be used to optimize PHB production.
ISSN:1615-7591
1615-7605
DOI:10.1007/s00449-022-02779-9