Piezotronic Effect-Assisted Photoelectrochemical Exosomal MicroRNA Monitoring Based on an Electron Donor Self-Supplying Strategy

Exosomal microRNAs (miRNAs) as newly emerging reliable and noninvasive biomarkers have demonstrated a significant function in early cancer diagnosis. Photoelectrochemical (PEC) biosensing has attracted unprecedented attention in exosomal miRNA monitoring due to its inherent advantages of both electr...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2022-10, Vol.94 (39), p.13522-13532
Main Authors: Wang, Yanhu, Yang, Mengchun, Ge, Shenguang, Wang, Xiao, Yu, Jinghua
Format: Article
Language:English
Subjects:
Analytical chemistry
Bioassays
Biomarkers
Biosensors
Carrier recombination
Chemistry
Copper sulfides
Current carriers
Electrochemistry
Heterojunctions
Hybrids
Hydrogen peroxide
Metal-organic frameworks
MicroRNAs
miRNA
Monitoring
Optics
Photoelectric effect
Piezoelectricity
Recombination
Sensitivity analysis
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Summary:Exosomal microRNAs (miRNAs) as newly emerging reliable and noninvasive biomarkers have demonstrated a significant function in early cancer diagnosis. Photoelectrochemical (PEC) biosensing has attracted unprecedented attention in exosomal miRNA monitoring due to its inherent advantages of both electrochemical and optical techniques; however, the severe charge carrier recombination greatly restricts the PEC assay performance. Herein, a high-sensitive PEC strategy assisted by the piezoelectric effect is designed based on Bi2WO6/Cu2S heterojunctions and implemented for the monitoring of exosomal miRNAs. The introduction of the piezoelectric effect enables promoted electron–hole transfer and separation, thereby improving the analytical sensitivity. In addition, a target reprogramming metal–organic framework-capped CaO2 (MOF@CaO2) hybrids is prepared, in which MOF@CaO2 being responsive to exosomal miRNAs induces exposure of the capped CaO2 to H2O and then triggers self-supplying of H2O2, which effectively suppresses the electron–hole recombination, giving rise to an amplified photocurrent and a decrease in the cost of the reaction. Benefiting from the coupled sensitization strategy, the as-fabricated PEC strategy exhibits high sensitivity, specificity, low cost, and ease of use for real-time analysis of exosomal miRNAs within the effectiveness linear range of 0.1 fM–1 μM. The present work demonstrates promising external field coupling-enhanced PEC bioassay and offers innovative thoughts for applying this strategy in other fields.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.2c02821