Development and characterization of microsatellite markers in the African timber tree species Cylicodiscus gabunensis (Fabaceae)

Background Cylicodiscus gabunensis (Fabaceae) or ‘Okan’ is a Central African multipurpose timber species that is used for heavy construction and traditional medicine. Despite being currently heavily exploited, the species shows a low population density and a natural regeneration deficit in dense for...

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Bibliographic Details
Published in:Molecular biology reports 2022-11, Vol.49 (11), p.11209-11214
Main Authors: Bhasin, Oriana B., Sergeant, Saskia, Hardy, Olivier J.
Format: Article
Language:English
Subjects:
Animal Anatomy
Animal Biochemistry
Biomedical and Life Sciences
Cylicodiscus gabunensis
Fabaceae
Forest management
Gene flow
Genetic diversity
Genetic markers
Genetic structure
Heavy construction
Heterozygosity
Histology
Inbreeding
Inbreeding depression
Life Sciences
Microsatellites
Morphology
Population density
Population genetics
Seed dispersal
Short Communication
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Summary:Background Cylicodiscus gabunensis (Fabaceae) or ‘Okan’ is a Central African multipurpose timber species that is used for heavy construction and traditional medicine. Despite being currently heavily exploited, the species shows a low population density and a natural regeneration deficit in dense forest. Methods and Results We aimed to characterize polymorphic microsatellite markers that can be used to study patterns of genetic structure and gene flow (mating system, pollen and seed dispersal and inbreeding depression) and ultimately, help to develop sustainable forest management practices. We developed 24 polymorphic markers that can be amplified in three PCR multiplexes that were tested in 647 individuals of C. gabunensis from three populations. The number of alleles ranged from 4 to 27 and the average observed and expected heterozygosity across loci and populations were Ho = 0.585 (0.081–0.936) and He = 0.510 (0.076–0.914), respectively. Conclusions This set of markers is a useful tool for exploring intra-specific diversity, genetic structure and gene flow of C. gabunensis.
ISSN:0301-4851
1573-4978
DOI:10.1007/s11033-022-07908-x