The protective effect of cilostazol on experimental ischemia/reperfusion injury in rats ovaries on in vitro fertilization outcomes

• Published in: Turkish journal of obstetrics and gynecology 2022-09, Vol.19 (3), p.236-241
• Main Authors: Budak, Özcan, Bostancı, Mehmet Süha, Köse, Osman, Çakıroğlu, Hüseyin, Durmaz, Özkan, Erdem Çokluk
• Format: Article
• Language: English
• Subjects: Abdomen; Embryos; In vitro fertilization; Infertility; Ischemia; Normal distribution; Ovaries; Ovulation; Sperm; Surgery; Sutures
• ISSN: 2149-9322; 2149-9330
• DOI: 10.4274/tjod.galenos.2022.29599

Objective: Ovarian torsion decreases ovarian reserve because of ischemic and reperfusion damage it causes. In this study, we investigated the protective effect of cilostazol (CIL) on experimental ischemia (I) and ischemic-reperfusion (I/R) damage in rat ovaries with in vitro fertilization (IVF) results. Materials and Methods: Forty-eight adult female Sprague-Dawley albino rats were randomly assigned to 6 groups with 8 animals in each group: Sham (S), I, I/R, S + CIL, I + CIL and I/R + CIL. The I groups were subjected to bilateral adnexal torsion for 3 h, while the I/R and I/R + CIL groups received subsequent detorsion for 3 h. Twenty-two mg/kg of CIL was given via oral gavage 30 min before surgery on the I (I+ CIL) or reperfusion (I/R + CIL) groups. Oocytes were collected before the IVF procedure and after ovulation induction with 150-300 IU/kg pregnant mare serum gonadotropin. Results: The metaphase oocytes reached their highest value of 4.73±0.96 in the S+ CIL group and reached their lowest value of 0.51±0.55 in the I/R group. There were statistically significant differences in the number of second-day embryos among the I, I+ CIL, and I/R and I/R+ CIL groups (p=0.000). When the groups were compared in terms of Anti-Müllerian hormone change, the highest decrease was observed in the I and I/R groups. Conclusion: CIL pretreatment before surgery has a protective effect against I and I/R in rats with ovarian torsion.