Pseudomonas sp. NGC7 as a microbial chassis for glucose-free muconate production from a variety of lignin-derived aromatics and its application to the production from sugar cane bagasse alkaline extract

• Published in: Bioresource technology 2022-09, Vol.359, p.127479-127479, Article 127479
• Main Authors: Akutsu, Miho, Abe, Nanase, Sakamoto, Chiho, Kurimoto, Yuki, Sugita, Haruka, Tanaka, Makoto, Higuchi, Yudai, Sakamoto, Kimitoshi, Kamimura, Naofumi, Kurihara, Hiroyuki, Masai, Eiji, Sonoki, Tomonori
• Format: Article
• Language: English
• Subjects: Alkaline extracts; aromatic compounds; cis,cis-Muconate; genes; hardwood; Lignin; Pseudomonas; Sugar cane bagasse; sugarcane bagasse; technology
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2022.127479

[Display omitted] •Pseudomonas sp. NGC7 was used as a host cell to produce muconate.•Muconate was produced from a variety of aromatics by tuning its metabolic flow.•The engineered strains produced muconate without using carbon other than aromatics.•Glucose-free muconate production from bagasse alkaline extract was demonstrated. cis,cis-Muconate (ccMA) is a promising platform for use in synthesizing various polymers. A glucose-free ccMA production using Pseudomonas sp. NGC7 from hardwood lignin-derived aromatic compounds was previously reported. In that system, syringyl nucleus compounds were essential for growth. Here, it is shown that NGC7 is available for glucose-free ccMA production even from a mixture of lignin-derived aromatics that does not contain syringyl nucleus compounds. By introducing a gene set for the protocatechuate (PCA)-shunt consisting of PCA 3,4-dioxygenase and PCA decarboxylase into an NGC7-derived strain deficient in PCA 3,4-dioxygenase and ccMA cycloisomerase, it was succeeded in constructing a ccMA-producing strain that grows on a lignin-derived aromatics mixture containing no syringyl nucleus compounds. Finally, it is demonstrated that the engineered strain produced ccMA from sugar cane bagasse alkaline extract in 18.7 mol%. NGC7 is thus shown to be a promising microbial chassis for biochemicals production from lignin-derived aromatics.