Functional identification of the DHN melanin synthesis gene cluster and its role in UV-C tolerance in citrus postharvest pathogenic fungus Penicillium digitatum

• Published in: Fungal biology 2022-09, Vol.126 (9), p.566-575
• Main Authors: Yang, Fan, Cheng, Lingtong, Du, Yuling, Xia, Lungang, Long, Chao-an
• Format: Article
• Language: English
• Subjects: albino; cell walls; Citrus; Citrus - microbiology; Citrus sinensis; conidia; conidiation; DHN melanin; Fungal Proteins - genetics; fungi; fungicide resistance; Gene Expression Regulation, Fungal; hydrophobicity; irradiation; melanin; Melanins - metabolism; Multigene Family; mutants; mycology; Penicillium; Penicillium digitatum; pigmentation; plant pathogens; Spores, Fungal; UV-C tolerance; virulence; virulent strains
• ISSN: 1878-6146; 1878-6162
• DOI: 10.1016/j.funbio.2022.07.002

The filamentous fungus Penicillium digitatum brings out great losses in citrus fruits by causing citrus green mold disease during the postharvest period. Previously, we obtained a T-DNA insertion mutant N2130 of P. digitatum, which produced albino conidia. To understand the role of green-grey conidial pigment in P. digitatum, we identified the insertion site and deeply explored the 1,8-dihydroxynaphtsalene (DHN)-melanin synthesis gene cluster in this phytopathogen. In this study, we deleted five genes in P. digitatum, PdPksP, PdAbr1, PdArp1, PdArp2, and PdAyg1, and the experiments were further performed on phenotype analyses, including pigmentation, UV-C tolerance, virulence, growth rate, conidiation, stress (osmotic-, oxidative-, cell wall disturbing-, and high temperature-) tolerance, fungicide resistance, and conidial hydrophobicity. The results showed that the five deletion mutants (ΔPdPksP, ΔPdAbr1, ΔPdArp1, ΔPdArp2 and ΔPdAyg1) produced albino, brownish, brown, reddish-brown, and Yellowish green conidia, respectively. In addition, the survival colony forming units (CFUs) of the deletion mutants, under the treatment of UV-C radiation (261.4 mJ/cm2), were 0.3- to 0.6-fold of those surviving in wild-type strain N1. Moreover, after 522.8 mJ/cm2-UV-C-irradiation on conidia, the deletion mutants showed a larger decrease in pathogenicity on Valencia Orange fruits compared with strain N1. However, there were no significant differences among other phenotypes tested in this study. Collectively, our research reported the DHN-melanin synthesis pathway in P. digitatum for the first time, and revealed that DHN-melanin is important for P. digitatum to tolerate UV-C irradiation.