Advancements in G protein‐coupled receptor biosensors to study GPCR‐G protein coupling

Enzymatic and cellular signalling biosensors are used to decipher the activities of complex biological systems. Biosensors for monitoring G protein‐coupled receptors (GPCRs), the most drugged class of proteins in the human body, are plentiful and vary in utility, form and function. Their application...

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Bibliographic Details
Published in:British journal of pharmacology 2023-06, Vol.180 (11), p.1433-1443
Main Authors: Olsen, Reid H. J., English, Justin G.
Format: Article
Language:English
Subjects:
BERKY
Biosensing Techniques
biosensor
Biosensors
BRET
FRET
functional selectivity
G protein coupled receptors
G proteins
GEMTA
GPCR
GTP-Binding Proteins - metabolism
Humans
Receptors, G-Protein-Coupled - metabolism
Signal Transduction
signalling bias
SPASM
transducerome
TRUPATH
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Summary:Enzymatic and cellular signalling biosensors are used to decipher the activities of complex biological systems. Biosensors for monitoring G protein‐coupled receptors (GPCRs), the most drugged class of proteins in the human body, are plentiful and vary in utility, form and function. Their applications have continually expanded our understanding of this important protein class. Here, we briefly summarize a subset of this field with accelerating importance: transducer biosensors measuring receptor‐coupling and selectivity, with an emphasis on sensors measuring receptor association and activation of heterotrimeric signalling complexes.
ISSN:0007-1188
1476-5381
DOI:10.1111/bph.15962