First characterization of the faecal resistome of eurasian otter (Lutra lutra), a sentinel species for aquatic environments

• Published in: Chemosphere (Oxford) 2022-12, Vol.309, p.136644-136644, Article 136644
• Main Authors: Dias, Diana, Fonseca, Carlos, Mendo, Sónia, Caetano, Tânia
• Format: Article
• Language: English
• Subjects: ARGs; E. coli; Enterococcus spp; MGEs; qPCR; Wildlife
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/j.chemosphere.2022.136644

Antimicrobial resistance (AMR) is a global health concern. Nowadays, antibiotic resistance genes (ARGs) are considered emerging pollutants. This study followed the One Health framework, in which AMR surveillance in the environment, including in wild animals, is advisable to mitigate this problem. Here we investigated AMR associated with Eurasian otter, a semi-aquatic mammal considered an indicator of freshwater health. To do so, otter's faecal resistome was characterized by a high-throughput qPCR array. This technique has a high-capacity of ARGs profiling. Additionally, we have assessed the antimicrobial susceptibility of two indicator bacteria, E. coli and Enterococcus spp, isolated from otter spraints and interpreted the results according to clinical and epidemiological cut-offs (ECOFFs). [Display omitted] •First study assessing otter faecal resistome using a high-throughput approach.•The most abundant classes of ARGs confer resistance to quinolones and β-lactams.•“High-threat” ARGs that might endanger human health have been identified.•High percentage of ciprofloxacin resistance was detected.•Few MDR isolates were identified.