The effects of polarized photobiomodulation on cellular viability, proliferation, mitochondrial membrane potential and apoptosis in human fibroblasts: Potential applications to wound healing

• Published in: Journal of photochemistry and photobiology. B, Biology Biology, 2022-11, Vol.236, p.112574, Article 112574
• Main Authors: Tripodi, Nicholas, Sidiroglou, Fotios, Fraser, Sarah, Husaric, Maja, Kiatos, Dimitrios, Apostolopoulos, Vasso, Feehan, Jack
• Format: Article
• Language: English
• Subjects: Apoptosis; Biological effects; Cell Proliferation; Cell viability; Cellular apoptosis; Cellular proliferation; Cellular viability; Chronic conditions; Chronic illnesses; Electric fields; Fibroblasts; Humans; Linear polarization; Low level light therapy; Membrane potential; Membrane Potential, Mitochondrial; Membranes; Mitochondria; Mitochondrial membrane potential; Oxidative stress; Photobiomodulation; Polarization; Polarized light; Wound Healing
• ISSN: 1011-1344; 1873-2682; 1873-2682
• DOI: 10.1016/j.jphotobiol.2022.112574

Photobiomodulation (PBM) is a widely used therapeutic intervention used to treat several chronic conditions. Despite this, fundamental research underpinning its effectiveness is lacking, highlighted by the lack of a definitive mechanism of action. Additionally, there are many treatment variables which remain underexplored, one of those being the effect of polarization the property of light that specifies the direction of the oscillating electric field. When applied to PBM, using linearly polarized light, when compared to otherwise identical non-polarized light, may enhance its biological efficacy. As such, we investigated the potential biological effects of polarized PBM when compared to non-polarized and non-irradiated controls in the domains of cellular viability, proliferation, apoptosis and mitochondrial membrane potential (ΔΨ) within cells exposed to oxidative stress. It was noted that polarized PBM, when compared to non-polarized PBM and non-irradiated controls, demonstrated mostly increased levels of cellular proliferation and ΔΨ, whilst decreasing the amount of cellular apoptosis. These results indicate that polarization may have utility in the clinical application of PBM. Future research is needed to further elucidate the underpinning mechanisms of PBM and polarization. •The fundamental underpinning mechanisms Photobiomodulation (PBM) remain elusive•Polarization of PBM presents as an attractive variable to investigate•Polarization appears to increase the efficacy of PBM comparted to matched PBM•Specifically it increased cellular proliferation and mitochondrial membrane potential•Polarization of PBM also decreased cellular apoptosis