Acetylation of NarL K188 and K192 is involved in regulating Escherichia coli anaerobic nitrate respiration

• Published in: Applied microbiology and biotechnology 2022-11, Vol.106 (21), p.7209-7221
• Main Authors: Cai, Shu-Shan, Zhang, Liu-Qing, Zhang, Qian, Ye, Bang-Ce, Zhou, Ying
• Format: Article
• Language: English
• Subjects: Acetyl phosphate; Acetylation; Acetyltransferase; Anaerobic respiration; Analysis; Applied Microbial and Cell Physiology; Binding; Biomedical and Life Sciences; Biotechnology; Deoxyribonucleic acid; DNA; DNA-ligand interactions; E coli; Energy conservation; Environmental changes; Escherichia coli; Flora; Gene regulation; Identification and classification; Intestinal microflora; Intestine; Life Sciences; Lysine; Lysine N-acetyltransferase; Microbial Genetics and Genomics; Microbiology; Nitrates; Phosphorylation; Properties; Proteins; Regulatory proteins; Respiration
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-022-12185-6

As a facultative anaerobe, Escherichia coli can activate various respiratory chains during anaerobic growth, among which the mode of anaerobic respiration with nitrate allows good energy conservation. NarL is one of the regulatory proteins in the Nar two-component system that regulates anaerobic respiration in E. coli . Previous studies have shown that NarL activates downstream gene regulation through phosphorylation. However, there are few studies on other protein translational modifications that influence the regulatory function of NarL. Herein, we demonstrate that acetylation modification exists on K188 and K192, the two lysine residues involved in contacting to DNA, and the degree of acetylation has significant effects on DNA-binding abilities, thus affecting the anaerobic growth of E. coli . In addition, NarL is mainly regulated by acetyl phosphate, but not by peptidyl-lysine N-acetyltransferase. These results indicate that non-enzymatic acetylation of NarL by AcP is one of the important mechanisms for the nitrate anaerobic respiratory pathway in response to environmental changes, which extends the idea of the mechanism underlying the response of intestinal flora to changes in the intestinal environment. Key points • Acetylation was found in NarL, which was mainly mediated by AcP. • Non-enzymatic acetylation at K188 and K192 affects NarL binding ability. • Acetylation of NarL K188 and K192 regulates anaerobic nitrate growth of E. coli.