MitoNEET’s Reactivity of Lys55 toward Pyridoxal Phosphate Demonstrates its Activity as a Transaminase Enzyme

• Published in: ACS chemical biology 2022-10, Vol.17 (10), p.2716-2722
• Main Authors: Kunk, Courtney, Kruger, Josh, Mendoza, George, Markitan, Joey, Bias, Taylor, Mann, Alexis, Nath, Abhinav, Geldenhuys, Werner J., Menze, Michael A., Konkle, Mary E.
• Format: Article
• Language: English
• Subjects: Cysteine; Glutamates; Histidine; Hydrogen - metabolism; Iron - metabolism; Iron-Sulfur Proteins - chemistry; Ketoglutaric Acids; Lysine; Mitochondrial Proteins - metabolism; Pyridoxal Phosphate - metabolism; Sulfur; Transaminases - metabolism
• ISSN: 1554-8929; 1554-8937
• DOI: 10.1021/acschembio.2c00572

MitoNEET is a [2Fe-2S] redox active mitochondrial protein belonging to the CDGSH iron–sulfur domain (CISD) family of proteins. MitoNEET has been implicated as a potential target for drug development to treat various disorders, including type-2 diabetes, cancer, and Parkinson’s disease. However, the specific cellular function(s) for mitoNEET still remains to be fully elucidated, and this presents a significant roadblock in rational drug development. Here, we show that mitoNEET binds the enzymatic cofactor pyridoxal phosphate (PLP) specifically at only one of its 11 lysine residues, Lys55. Lys55 is part of the soluble portion of the protein and is in a hydrogen-bonding network with the histidine residue that ligates the [2Fe-2S] cluster. In the presence of mitoNEET, PLP catalyzes the transamination reaction of the amino acid cysteine and the alpha-keto acid 2-oxoglutarate to form 3-mercaptopyruvate and glutamate. This work identifies, for the first time, mitoNEET as an enzyme with cysteine transaminase activity.