Roles of MEF2A and MyoG in the transcriptional regulation of bovine LATS2 gene

• Published in: Research in veterinary science 2022-12, Vol.152, p.417-426
• Main Authors: Zhang, Jiupan, Raza, Sayed Haidar Abbas, Wei, Dawei, Yaping, Song, Chao, Jiang, Jin, Wang, Almohaimeed, Hailah M., A Batarfi, Munirah, Assiri, Rasha, Aggad, Waheeb S., Ghalib, Samirah H., Ageeli, Abeer A.
• Format: Article
• Language: English
• Subjects: Bovine; cattle; cell proliferation; chromatin immunoprecipitation; computer software; Expression; infancy; LATS2 gene; liver; longissimus muscle; luciferase; myoblasts; myotubes; promoter regions; tissue repair; transcription (genetics); Transcriptional regulation; tumor suppressor genes; veterinary medicine
• ISSN: 0034-5288; 1532-2661
• DOI: 10.1016/j.rvsc.2022.08.030

As an important downstream effector gene in the hippo signaling pathway, large tumor suppressor gene 2 (LATS2) is involved in cell proliferation and differentiation, organ size and tissue regeneration, and plays an important role in regulating the growth and development of animal muscles. The purpose of this study is to explore the temporal expression of bovine LATS2 gene, and determine the key transcription factors for regulating bovine LATS2 gene. The result showed that bovine LATS2 gene was highly expressed in liver and longissimus dorsi, and was up-regulated in infancy muscle. In addition, it was highly expressed on the 2th day during the differentiation stage of myoblast. The upstream 1.7 Kb sequence of the 5 ‘translation region of bovine LATS2 gene was cloned, and 7 different deletion fragments were amplified by the upstream primers. These fragments were constructed into double luciferase reporter vectors and transfected into myoblasts and myotubes cells, respectively to detect the core promoter regions. In addition, the key transcription factors of the core promoter sequence of the bovine LATS2 gene were analyzed and predicted by online software. Combining with site-directed mutations, siRNA interference and chromatin immunoprecipitation technology, it was identified that MEF2A and MyoG combined in core promoter region (−248/−56) to regulate the transcription activity of bovine LATS2 gene. The results have laid a theoretical foundation for exploring the molecular regulation mechanism of LATS2 gene in the process of muscle growth. •Bovine LATS2 was highly expressed in the longissimus thoracis and undifferentiated myoblast of Qinchuan cattle.•The expression pattern of LATS2 gene at mRNA and protein levels showed the same trend.•The core promoter of bovine LATS2 was located within the region −248/−58 relative to the TSS.•MEF2Aand MyoG bind to the proximal minimal promoter of LATS2, and plays an important role in its transcriptional regulation.