Co-expression of Xylose Transporter and Fructose-Bisphosphate Aldolase Enhances the Utilization of Xylose by Lactococcus lactis IO-1

The raw material cost of lactic acid fermentation accounts for the main part of the production cost, and this necessitates the exploration of the efficient use of cheap raw materials in lactic acid production. We compared the outcomes of the homologous expressions of xylose transporters ( xylFGH , x...

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Published in:Applied biochemistry and biotechnology 2023-02, Vol.195 (2), p.816-831
Main Authors: Qiu, Yejuan, Qiu, Zhongyang, Xia, Jun, Liu, Xiaoyan, Zhang, Hanwen, Yang, Yuxiang, Hou, Wenyi, Li, Xiangqian, He, Jianlong
Format: Article
Language:English
Subjects:
6-Phosphofructokinase
Acid production
Acids
Aldolase
Biochemistry
Biotechnology
Chemistry
Chemistry and Materials Science
Fermentation
Fructose
Fructose-bisphosphate aldolase
Fructose-Bisphosphate Aldolase - genetics
Fructose-Bisphosphate Aldolase - metabolism
Gene expression
Genes
Lactic Acid
Lactococcus lactis
Lactococcus lactis - genetics
Metabolic pathways
Metabolism
Original Article
Phosphofructokinase
Production costs
Raw materials
Xylose
Xylose - metabolism
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Summary:The raw material cost of lactic acid fermentation accounts for the main part of the production cost, and this necessitates the exploration of the efficient use of cheap raw materials in lactic acid production. We compared the outcomes of the homologous expressions of xylose transporters ( xylFGH , xylE , araE , and xylT ), 6-phosphofructokinase ( pfkA ), fructose-bisphosphate aldolase ( fbaA ), and their co-expression in Lactococcus lactis IO-1 on lactic acid production using xylose as the raw material. We found that the production rate of lactic acid on xylose fermentation by L. lactis IO-1 overexpressing fbaA was the highest (14.42%). Among the xylose transporters investigated, XylT had the strongest xylose transport capacity in L. lactis IO-1, with an increase in the lactic acid production rate by 10.38%. The genes near the overexpression of fbaA or xylT in the metabolic pathway were more upregulated than the distant genes. The co-expression of fbaA and xylT increased the production rate of lactic acid by 27.84% on xylose fermentation by L. lactis IO-1. This work presents a novel strategy for the simultaneous enhancement of the expression of important genes at the beginning and midway of the xylose metabolic pathway of L. lactis IO-1, which could greatly improve the target production. Graphical abstract
ISSN:0273-2289
1559-0291
DOI:10.1007/s12010-022-04168-0