Discerning Comparison of 1 and 0.5% Ethylene Glycol in Sprague-Dawley Rats with Modeled Urolithiasis

A common method of modeling urolithiasis is the use of 1 and 0.75% ethylene glycol, or a combination of ethylene glycol with other lithogens, but too rapid progression of the disease and multiple organ toxicity have been reported. We developed a urolithiasis model in Sprague-Dawley rats, in which th...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2022-09, Vol.173 (5), p.673-676
Main Authors: Bervinova, A. V., Borozdina, N. A., Palikov, V. A., Palikova, Yu. A., Mikhailov, E. S., Kravchenko, I. N., Rykov, V. A., Ponomareva, T. I., Semushina, S. G., Pakhomova, I. A., Dyachenko, I. A., Murashev, A. N.
Format: Article
Language:English
Subjects:
Animal experimentation
Animals
Biomedical and Life Sciences
Biomedicine
Blood
Calculi, Urinary
Cell Biology
Cholesterol
Creatinine
Diuresis
Ethylene
Ethylene glycol
Ethylenediaminetetraacetic acid
Hematocrit
Internal Medicine
Laboratory Medicine
Leukocytes
Lithiasis
Medical examination
Pathology
Rodents
Toxicity
Triglycerides
Urinary tract diseases
Urine
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Summary:A common method of modeling urolithiasis is the use of 1 and 0.75% ethylene glycol, or a combination of ethylene glycol with other lithogens, but too rapid progression of the disease and multiple organ toxicity have been reported. We developed a urolithiasis model in Sprague-Dawley rats, in which the animals received a relatively low concentration of ethylene glycol (0.5%), but for a long-term period (6 weeks) followed by animal observation during the 6-week recovery period. In urine samples, signs of the urolithiasis development were observed starting from the sixth week: the presence of ketones, decrease in diuresis and urine pH; in the blood, urea, protein, and hematocrit were elevated. However, no leukocytes were detected in the urine; in the blood, no shifts in differential leukocyte count and no elevation in ALT, creatinine, cholesterol, and triglycerides were observed, which indicates the absence of multiple organ failure while using 1% ethylene glycol. In addition, the animals receiving 0.5% ethylene glycol were followed up to 12 weeks in contrast to animals receiving 1% ethylene glycol (the experiment in this case was stopped during the third week for ethical reasons).
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-022-05611-2