Human embryonic stem cell-specific role of YAP in maintenance of self-renewal and survival

Human embryonic stem cells (hESCs) have unique characteristics, such as self-renewal and pluripotency, which are distinct from those of other cell types. These characteristics of hESCs are tightly regulated by complex signaling mechanisms. In this study, we demonstrate that yes-associated protein (Y...

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Published in:Cellular and molecular life sciences : CMLS 2022-11, Vol.79 (11), p.544-544, Article 544
Main Authors: Choe, Mu Seog, Bae, Chang Min, Kim, So Jin, Oh, Seung Tack, Kown, Yu Jin, Choi, Won-young, Han, Ho Jae, Baek, Kyung Min, Chang, Woochul, Kim, Joong Sun, Lim, Kyung Seob, Yun, Seung Pil, Lee, Min Young
Format: Article
Language:English
Subjects:
Antibiotics
Biochemistry
Biomedical and Life Sciences
Biomedicine
Cell Biology
Cell self-renewal
Cell survival
Doxycycline
Embryo cells
Life Sciences
Maintenance
Original Article
Pluripotency
Stem cells
Survival
Yes-associated protein
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Summary:Human embryonic stem cells (hESCs) have unique characteristics, such as self-renewal and pluripotency, which are distinct from those of other cell types. These characteristics of hESCs are tightly regulated by complex signaling mechanisms. In this study, we demonstrate that yes-associated protein (YAP) functions in an hESC-specific manner to maintain self-renewal and survival in hESCs. hESCs were highly sensitive to YAP downregulation to promote cell survival. Interestingly, hESCs displayed dynamic changes in YAP expression in response to YAP downregulation. YAP was critical for the maintenance of self-renewal. Additionally, the function of YAP in maintenance of self-renewal and cell survival was hESC-specific. Doxycycline upregulated YAP in hESCs and attenuated the decreased cell survival induced by YAP downregulation. However, decreased expression of self-renewal markers triggered by YAP downregulation and neural/cardiac differentiation were affected by doxycycline treatment. Collectively, the results reveal the mechanism underlying the role of YAP and the novel function of doxycycline in hESCs.
ISSN:1420-682X
1420-9071
DOI:10.1007/s00018-022-04558-x