Heterojunction MnO2-nanosheet-decorated Ag nanowires with enhanced oxidase-like activity for the sensitive dual-mode detection of glutathione

The biocatalytic design of nanomaterials with enzyme-like activity is considered a reliable and promising toolkit for the generation of diagnostic agents in complex biological microenvironments. However, the preparation of nanomaterials while maintaining a high catalytic activity in tumor cells (pH...

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Bibliographic Details
Published in:Nanoscale 2022-10, Vol.14 (41), p.15340-15347
Main Authors: Lin, Tian, Huang, Zijun, Weidan Na, Liu, Yuanyuan, Wang, Shuai, He, Yu, Cheng, Wenjing, Huang, Tianzi, Zhao, Li, Li, Tongxiang
Format: Article
Language:English
Subjects:
Biomimetics
Biosensors
Catalytic activity
Colorimetry
Enzymes
Glutathione
Heterojunctions
Hydrogen peroxide
Manganese dioxide
Nanomaterials
Nanosheets
Nanowires
Oxidase
Oxidation
Redox reactions
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Summary:The biocatalytic design of nanomaterials with enzyme-like activity is considered a reliable and promising toolkit for the generation of diagnostic agents in complex biological microenvironments. However, the preparation of nanomaterials while maintaining a high catalytic activity in tumor cells (pH 6.0–6.5) poses a prominent challenge. Herein, we constructed a biomimetic enzyme-trigged dual-mode system with colorimetry at 652 nm and photothermal biosensors to detect glutathione based on hollow MnO2-nanosheet-decorated Ag nanowires (Ag@MnO2) as an oxidase-like nanozyme. As expected, Ag@MnO2 catalyzed the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) in the absence of H2O2, leading to a blue-colored oxidized TMB (oxTMB) that displayed oxidase-like activity in pH 6.0. Interestingly, the portable dual-mode colorimetry and photothermal method for GSH was developed based on the redox reaction between GSH and oxTMB. This detection method exhibited a wide linear range of 0.1–55 μM for GSH with a low detection limit of 0.08 μM. This work highlights a new insight into nanotechnology by taking advantage of biomimetic design in biological analysis.
ISSN:2040-3364
2040-3372
DOI:10.1039/d2nr04294k