Accumulation of Galactinol and ABA Is Involved in Exogenous EBR-Induced Drought Tolerance in Tea Plants

• Published in: Journal of agricultural and food chemistry 2022-10, Vol.70 (41), p.13391-13403
• Main Authors: Zhang, Yongheng, Xiao, Yezi, Zhang, Yingao, Dong, Yuan, Liu, Yingqing, Liu, Lu, Wan, Siqing, He, Jingyuan, Yu, Youben
• Format: Article
• Language: English
• Subjects: Food and Beverage Chemistry/Biochemistry
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/acs.jafc.2c04892

Drought stress severely limits growth and causes losses in the yield of tea plants. Exogenous application of 24-epibrassinolide (EBR) positively regulates drought responses in various plants. However, whether EBR could contribute to drought resistance in tea plants and the underlying mechanisms has not been investigated. Here, we found that EBR application is beneficial for the drought tolerance of tea plants. The transcriptome results revealed that EBR could contribute to tea plant drought resistance by promoting galactinol and abscisic acid (ABA) biosynthesis gene expression. The content of galactinol was elevated by EBR and EBR-responsive CsDof1.1 positively regulated the expression of the galactinol synthase genes CsGolS2-1 and CsGolS2-2 to contribute to the accumulation of galactinol by directly binding to their promoters. Moreover, exogenous EBR was found to elevate the expression of genes related to ABA signal transduction and stomatal closure regulation, which resulted in the promotion of stomatal closure. In addition, EBR-responsive CsMYC2-2 is involved in ABA accumulation by binding to the promoters CsNCED1 and CsNCED2 to activate their expression. In summary, findings in this study provide knowledge into the transcriptional regulatory mechanism of EBR-induced drought resistance in tea plants.