Eight TRIM32 isoforms from oriental river prawn Macrobrachium nipponense are involved in innate immunity during white spot syndrome virus infection

• Published in: Fish & shellfish immunology 2022-12, Vol.131, p.368-380
• Main Authors: Chen, Wei-yu, Gong, Yi-qing, Zhou, Xu-ri, Zhang, Rui-dong, Liu, Song-hai, Lu, Wei, Ren, Qian, Huang, Ying
• Format: Article
• Language: English
• Subjects: Animals; Arthropod Proteins - genetics; Arthropod Proteins - metabolism; DsRNA interference; Expression regulation; Gene Expression Regulation; Immunity, Innate - genetics; Innate immune response; Macrobrachium nipponense; Palaemonidae; Phylogeny; Protein Isoforms - genetics; Protein Isoforms - metabolism; Tripartite motif proteins; Tripartite Motif Proteins - genetics; Tripartite Motif Proteins - metabolism; White spot syndrome virus; White spot syndrome virus 1 - physiology
• ISSN: 1050-4648; 1095-9947
• DOI: 10.1016/j.fsi.2022.10.019

Tripartite motif (TRIM) proteins comprise a large family of RING-type ubiquitin E3 ligases that regulate important biological processes. In this study, full-length MnTRIM32 cDNA was obtained from oriental river prawn Macrobrachium nipponense, and eight MnTRIM32 isoforms generated by alternative splicing were identified. The open reading frames of the eight MnTRIM32 isoforms were predicted to be separately composed of 402, 346, 347, 346, 414, 358, 359, and 358 amino acid residues. Protein structural analysis revealed that all MnTRIM32 isoforms contained a RING domain and a coiled coil region. MnTRIM32 was ubiquitously expressed in all tissues tested, with the highest expression in the hepatopancreas. The mRNA levels of MnTRIM32 in the gills, stomach, and intestine of prawns were found to undergo time-dependent enhancement following white spot syndrome virus (WSSV) stimulation. Double-stranded RNA interference studies revealed that MnTRIM32 silencing significantly downregulated the expression levels of interferon (IFN) regulatory factor MnIRF, IFN-like factor MnVago4, and tumor necrosis factor MnTNF. Furthermore, knockdown of MnTRIM32 in WSSV-challenged prawns increased the expression of VP28 and the number of WSSV copies, suggesting that MnTRIM32 plays a positive role in limiting WSSV infection. These findings provided strong evidence for the important role of MnTRIM32 in the antiviral innate immunity of M. nipponense. •Eight TRIM32 isoforms were identified from prawn Macrobrachium nipponense.•Eight MnTRIM32 isoforms were generated by alternative splicing.•MnTRIM32 was significantly upregulated after white spot syndrome virus stimulation.•MnTRIM32 was involved in regulating the expression of MnIRF, MnVago4, and MnTNF.•Knockdown of MnTRIM32 increased the expression of VP28 and the number of WSSV copies.