Stress-responses of microbes in oil reservoir under high tetracycline exposure and their environmental risks

• Published in: Environmental pollution (1987) 2022-12, Vol.315, p.120355-120355, Article 120355
• Main Authors: Yun, Yuan, Su, Tianqi, Gui, Ziyu, Tian, Xuefeng, Chen, Yu, Cao, Yunke, Yang, Shicheng, Xie, Jinxia, Anwar, Nusratgul, Li, Mingchang, Li, Guoqiang, Ma, Ting
• Format: Article
• Language: English
• Subjects: Antibiotic resistance genes; Antibiotics; Ecosystem functioning; HGT; Microbial community; Oil reservoir
• ISSN: 0269-7491; 1873-6424
• DOI: 10.1016/j.envpol.2022.120355

As the groundwater ecosystem is connected with surface, antibiotics and antibiotic resistance genes (ARGs) in aquatic environments will gradually infiltrate into the deep environment, posing a potential threat to groundwater ecosystem. However, knowledge on the environmental risk of antibiotics and ARGs in groundwater ecosystem and their ecological process still remains unexplored. In this study, lab-scale oil reservoirs under high tetracycline stress were performed to evaluate the dynamics of microbial communities, ARGs and potential functions by using 16S rRNA gene sequencing and metagenomics analysis. Although the presence of antibiotics remarkably reduced the microbial abundance and diversity in a short term, but remain stable or even increased after a long-term incubation. Antibiotic stress caused a greater diversity and abundance of ARGs, and higher numbers of ARGs-related species with the capacity to transfer ARGs to other microbes through horizontal gene transfer. Thus, a much more frequent associations of microbial community at both node- and network-level and a selective pressure on enrichment of antibiotic resistant bacteria related to “anaerobic n-alkane degradation” and “methylotrophic methanogenesis” were observed. It is important to emphasize that high antibiotic stress could also prevent some microbes related to “Sulfate reduction”, “Fe(II) oxidation”, “Nitrate reduction”, and “Xylene and Toluene degradation”. This study provides an insight into the long-term stress-responses of microbial communities and functions in oil reservoir under tetracycline exposure, which may help to elucidate the effect of antibiotic stress on biogeochemical cycling with microbial involvement in groundwater ecosystem. [Display omitted] •High tetracycline exposure caused dramatic changes of microbes in deep oil reservoir.•The abundance and diversity of microbes tended to increase under long-term stress.•Antibiotic stress caused an increase in the contribution of HGTs.•Microbial communities under antibiotic stress exhibited more frequent associations.•The dramatic reduction of S, Fe, N, aromatics-related cycles should be taken seriously.