Validation of laser doppler flowmetry to measure dermal blood flow of the pinnae in dogs with pinnal alopecia

Background Laser Doppler flowmetry (LDF) is a noninvasive method of measuring regional blood flow in humans. However, this method has not been widely applied to measure blood flow in dogs. Hypothesis/Objectives We hypothesised that LDF can measure changes in blood flow in canine pinnae accurately. T...

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Bibliographic Details
Published in:Veterinary dermatology 2023-02, Vol.34 (1), p.70-76
Main Authors: Ide, Kaori, Tokunaga, Makoto, Hiratsuka, Shogo, Iwasaki, Toshiroh, Nishifuji, Koji
Format: Article
Language:English
Subjects:
Alopecia
Alopecia - chemically induced
Alopecia - veterinary
Animals
Atropine
Atropine Derivatives
Baldness
Blood flow
Bucladesine
Cyclic AMP
dermal blood flow
dog
Dog Diseases - chemically induced
Dogs
Doppler effect
Drug dosages
Hemodynamics
Humans
Injection
Ischemia
laser doppler flowmetry
Laser-Doppler Flowmetry - methods
Laser-Doppler Flowmetry - veterinary
Lasers
Medetomidine
Skin
Skin diseases
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Summary:Background Laser Doppler flowmetry (LDF) is a noninvasive method of measuring regional blood flow in humans. However, this method has not been widely applied to measure blood flow in dogs. Hypothesis/Objectives We hypothesised that LDF can measure changes in blood flow in canine pinnae accurately. The objectives were to determine whether LDF could accurately detect dermal blood flow changes in canine pinnae caused by haemodynamic drugs and characterize the dermal blood flow in dogs with pinnal alopecia. Animals Sixteen laboratory‐owned healthy dogs, 25 client‐owned healthy control dogs and six dogs with pinnal alopecia suspected to be secondary to ischaemic dermatoses. Materials and Methods Clinical doses of the haemodynamic drugs atropine, medetomidine and dibutyryl cyclic adenosine monophosphate (dBcAMP), as well as topical dBcAMP, were administered to healthy beagles. Subsequently, an LDF apparatus was attached to the pinnae to analyse changes in dermal blood flow. Finally, LDF was used to measure auricular dermal blood flow in dogs with pinnal alopecia compared to healthy dogs. Results Dermal blood flow increased after atropine injection, during dBcAMP infusion and after topical dBcAMP ointment application, and decreased after medetomidine injection. Auricular dermal blood flow (in mL/min/100 g tissue) was significantly (p 
ISSN:0959-4493
1365-3164
DOI:10.1111/vde.13126