Kif26b contributes to the progression of interstitial fibrosis via migration and myofibroblast differentiation in renal fibroblast

• Published in: The FASEB journal 2022-11, Vol.36 (11), p.e22606-n/a
• Main Authors: Yamamura, Yuta, Iwata, Yasunori, Furuichi, Kengo, Kato, Takahiro, Yamamoto, Naoki, Horikoshi, Keisuke, Ogura, Hisayuki, Sato, Koichi, Oshima, Megumi, Nakagawa, Shiori, Miyagawa, Taro, Kitajima, Shinji, Toyama, Tadashi, Hara, Akinori, Sakai, Norihiko, Shimizu, Miho, Horike, Shinichi, Daikoku, Takiko, Nishinakamura, Ryuichi, Wada, Takashi
• Format: Article
• Language: English
• Subjects: Actins - genetics; Actins - metabolism; Adenine - metabolism; Animals; Cell Differentiation; Cell Movement; Collagen - metabolism; Connective Tissue Growth Factor - genetics; Connective Tissue Growth Factor - metabolism; Fibroblasts - metabolism; Fibrosis; Kidney - metabolism; Kif26b; Kinesins - genetics; Mice; Myh9; Myofibroblasts - metabolism; Myosin Heavy Chains - genetics; Myosin Heavy Chains - metabolism; nonmuscle myosin heavy chain II; renal fibroblast; renal fibrosis
• ISSN: 0892-6638; 1530-6860
• DOI: 10.1096/fj.202200355R

Kinesin family member 26b (Kif26b) is essential for kidney development, and its deletion in mice leads to kidney agenesis. However, the roles of this gene in adult settings remain elusive. Thus, this study aims to investigate the role of Kif26b in the progression of renal fibrosis. A renal fibrosis model with adenine administration using Kif26b heterozygous mice and wild‐type mice was established. Renal fibrosis and the underlying mechanism were investigated. The underlying pathways and functions of Kif26b were evaluated in an in vitro model using primary renal fibroblasts. Kif26b heterozygous mice were protected from renal fibrosis with adenine administration. Renal expressions of connective tissue growth factor (CTGF) and myofibroblast accumulation were reduced in Kif26b heterozygous mice. The expression of nonmuscle myosin heavy chain II (NMHCII), which binds to the C‐terminus of Kif26b protein, was also suppressed in Kif26b heterozygous mice. The in vitro study revealed reduced expressions of CTGF, α‐smooth muscle actin, and myosin heavy chain 9 (Myh9) via transfection with siRNAs targeting Kif26b in renal fibroblasts (RFB). RFBs, which were transfected by the expression vector of Kif26b, demonstrated higher expressions of these genes than non‐transfected cells. Finally, Kif26b suppression and NMHCII blockage led to reduced abilities of migration and collagen gel contraction in renal fibroblasts. Taken together, Kif26b contributes to the progression of interstitial fibrosis via migration and myofibroblast differentiation through Myh9 in the renal fibrosis model. Blockage of this pathway at appropriate timing might be a therapeutic approach for renal fibrosis.