BmGr4 responds to sucrose and glucose and expresses in tachykinin-related peptide-secreting enteroendocrine cells

The regulatory hormones known as tachykinin-related peptides (TRPs) are identified as brain-gut peptides in insects. Dietary components from mulberry leaves, including glucose, induce secretion of TRPs from Bombyx mori midgut. However, the sensory molecules that recognize these compounds are still u...

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Bibliographic Details
Published in:Insect biochemistry and molecular biology 2022-11, Vol.150, p.103858-103858, Article 103858
Main Authors: Mang, Dingze, Mayu, Kasubuchi, Toyama, Tomoko, Yamagishi, Takayuki, Sato, Ryoichi
Format: Article
Language:English
Subjects:
Animals
Bombyx - metabolism
Bombyx mori
Brain
Enteroendocrine cell
Enteroendocrine Cells - metabolism
Glucose - metabolism
Gustatory receptor
Larva - metabolism
Midgut
Neurosecretory cell
Sucrose - metabolism
Sucrose - pharmacology
Tachykinin-related peptides
Tachykinins - metabolism
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Summary:The regulatory hormones known as tachykinin-related peptides (TRPs) are identified as brain-gut peptides in insects. Dietary components from mulberry leaves, including glucose, induce secretion of TRPs from Bombyx mori midgut. However, the sensory molecules that recognize these compounds are still unknown. Here, we identified the gustatory receptor, BmGr4, as a sucrose and glucose receptor using Ca2+ imaging. Immunostaining revealed BmGr4 expression not only in the midgut, but also in the brain. In addition, BmGr4 expression was found to co-localize with TRP-expressing cells in both midgut enteroendocrine cells (EECs) and brain neurosecretory cells (NSCs). Furthermore, dietary nutrients after food intake result in an increase of TRP-level in hemolymph of silkworm larvae. These results provide significant circumstantial evidence for the involvement of the sucrose and glucose receptor, BmGr4, in the elicitation of TRP secretion in midgut EECs and brain NSCs. [Display omitted] •BmGr4 was identified as a sucrose and glucose receptor using Ca2+ imaging.•BmGr4 is expressed in cells of anterior midgut, middle midgut, and brain of Bombyx mori larvae.•BmGr4 and tachykinin-related peptides co-expressed in midgut enteroendocrine cells and brain neurosecretory cells.•TRPs are secreted soon after the arrival of ingested food to the place of BmGr4 expressing cells in the midgut.
ISSN:0965-1748
1879-0240
DOI:10.1016/j.ibmb.2022.103858