Simultaneous quantification of six major allergens in commercial foods for children using a multiplex array on a digital versatile disc

•Simple, cost-effective optoelectrical microanalytical system was developed.•Simultaneous quantification of six food allergens was performed.•Biosensing platform offered appropriate sensitivity and specificity.•Analysis of 20 samples was achieved in 70 min.•Declared allergens in commercial food samp...

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Bibliographic Details
Published in:Food chemistry 2023-03, Vol.404, p.134570-134570, Article 134570
Main Authors: Sena-Torralba, Amadeo, Smits, Nathalie G.E., Blázquez, David, Albero-Pérez, Clara, Pallás-Tamarit, Yeray, Maquieira, Ángel, Morais, Sergi
Format: Article
Language:English
Subjects:
allergenicity
almonds
cost effectiveness
food chemistry
food production
Food-borne allergens
hazelnuts
Immunoassay
Microarray
microarray technology
milk
Multiplexing
Optoelectrical biosensor
peanuts
probiotics
seafoods
soybeans
wheat
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Summary:•Simple, cost-effective optoelectrical microanalytical system was developed.•Simultaneous quantification of six food allergens was performed.•Biosensing platform offered appropriate sensitivity and specificity.•Analysis of 20 samples was achieved in 70 min.•Declared allergens in commercial food samples were accuracy identified. The hypothesis of this study is centered around the logic that an enhanced analysis of potential allergens during the food production can lead to increased accuracy and reliability of food labeling. The development of a cost-effective and straightforward optoelectrical microanalytical system for the simultaneous quantification of the six most common food allergens (peanut, hazelnut, almond, milk, wheat, and soybean) is presented. The system uses a regular versatile disc (DVD) functionalized with highly selective antibodies in a microarray format and a DVD drive as the optical detector. The multiplexed assay reliably (RSD 
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2022.134570