The growth-regulating factor PdbGRF1 positively regulates the salt stress response in Populus davidiana × P. bolleana

• Published in: Plant science (Limerick) 2023-01, Vol.326, p.111502-111502, Article 111502
• Main Authors: Liu, Zhong-Yuan, Han, Ya-Ting, Wang, Chun-Yao, Lei, Xiao-Jin, Wang, Yuan-Yuan, Dong, Wen-Fang, Xie, Qing-Jun, Fu, Yu-Jie, Gao, Cai-Qiu
• Format: Article
• Language: English
• Subjects: DRE; Gene Expression Regulation, Plant; Hydrogen Peroxide - metabolism; PdbGRF1 transcription factor; Plant Proteins - genetics; Plant Proteins - metabolism; Plants, Genetically Modified - genetics; Populus - metabolism; Populus davidiana x P. bolleana; Salt Stress; Stress, Physiological - genetics
• ISSN: 0168-9452; 1873-2259
• DOI: 10.1016/j.plantsci.2022.111502

Growth-regulating factor (GRF) is a transcription factor unique to plants that plays a crucial role in the growth, development and stress adaptation of plants. However, information on the GRFs related to salt stress in Populus davidiana × P. bolleana is lacking. In this study, we characterized the activity of PdbGRF1 in transgenic Populus davidiana × P. bolleana under salt stress. qRTPCR analyses showed that PdbGRF1 was highly expressed in young leaves and that the pattern of PdbGRF1 expression was significantly changed at most time points under salt stress, which suggests that PdbGRF1 expression may be related to the salt stress response. Moreover, PdbGRF1 overexpression enhanced tolerance to salt stress. A physiological parameter analysis showed that the overexpression of PdbGRF1 significantly decreased the contents of hydrogen peroxide (H2O2) and malondialdehyde (MDA) and increased the activities of antioxidant enzymes (SOD and POD) and the proline content. A molecular analysis showed that PdbGRF1 regulated the expression of PdbPOD17 and PdbAKT1 by binding to the DRE (‘A/GCCGAC’) in their respective promoters. Together, our results demonstrate that the binding of PdbGRF1 to DRE regulates genes related to stress tolerance and activates the associated physiological pathways, and these effects increase the ROS scavenging ability, reduce the degree of damage to the plasma membrane and ultimately enhance the salt stress response in Populus davidiana × P. bolleana. •PdbGRF1 acts as a transcriptional activator to enhance salt stress tolerance by regulating PdbPOD17 and PdbAKT1.