Effect of platelet-rich plasma on the attachment of periodontal ligament fibroblasts to the diseased root surface and the attendant collagen formation

Objective To investigate the effect of different concentrations of platelet-rich plasma (PRP) on collagen formation via periodontal ligament fibroblasts (PDLFs) on the surface of demineralised diseased tooth roots. Methods Various PDLFs were grown from tissue explants, with the cells between the fif...

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Published in:Clinical oral investigations 2023-02, Vol.27 (2), p.529-539
Main Authors: Qian, Yi, Qian, Xueshen, Xu, Xiao-Bo
Format: Article
Language:English
Subjects:
Acetic acid
Cell culture
Collagen
Collagen - pharmacology
Dental roots
Dentistry
Dextrose
Edetic acid
Edetic Acid - pharmacology
Explants
Fibroblasts
Humans
Ligaments
Medicine
Optical density
Original Article
Periodontal Ligament
Platelet-Rich Plasma
Platelets
Statistical analysis
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Summary:Objective To investigate the effect of different concentrations of platelet-rich plasma (PRP) on collagen formation via periodontal ligament fibroblasts (PDLFs) on the surface of demineralised diseased tooth roots. Methods Various PDLFs were grown from tissue explants, with the cells between the fifth and eighth passage in the culture used. Human whole blood obtained from healthy subjects was collected in tubes containing an anticoagulant (acid-citrate-dextrose) and centrifuged (1300 rpm for 10 min) before the supernatant PRP layer was removed. A second spin at (2000 rpm for 10 min) produced the PRP fraction. The effect of PRP of various concentrations on the attachment of PDLFs on the diseased root surface of human teeth demineralised with ethylenediaminetetraacetic acid (EDTA) and treated with the PRP was then investigated in terms of PRP collagen formation, with the formation observed using the Sirius red staining method. Results The optical density values of the experimental groups were statistically significantly higher than those of the control groups ( P  
ISSN:1436-3771
1432-6981
1436-3771
DOI:10.1007/s00784-022-04748-8