In Vivo Simultaneous Imaging of Plasma Membrane and Lipid Droplets in Hepatic Steatosis using Red-Emissive Two-Photon Probes

The plasma membrane, which is a phosphoglyceride bilayer at the outer edge of the cell, plays diverse and important roles in biological systems. Visualization of the plasma membrane in live samples is important for various applications in biological functions. We developed an amphiphilic two-photon...

Full description

Saved in:
Bibliographic Details
Published in:Analytical chemistry (Washington) 2022-11, Vol.94 (43), p.15100-15107
Main Authors: Lee, Hyo Won, Pati, Tanmay Kumar, Lee, In-Jeong, Lee, Jeong-Mi, Kim, Bo Ra, Kwak, Sun Young, Kim, Hwan Myung
Format: Article
Language:English
Subjects:
Absorption cross sections
Chemistry
Droplets
Fatty liver
Fluorescence
Fluorescent indicators
Imaging
Lipids
Membranes
Photons
Selectivity
Steatosis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The plasma membrane, which is a phosphoglyceride bilayer at the outer edge of the cell, plays diverse and important roles in biological systems. Visualization of the plasma membrane in live samples is important for various applications in biological functions. We developed an amphiphilic two-photon (TP) fluorescent probe (THQ-Mem) to selectively monitor the plasma membrane in live samples. This probe exhibited red emission (620–700 nm), large TP absorption cross sections (δmax > 790 GM), and high selectivity to the plasma membrane. In cultured cells and in vivo hepatic tissue imaging, THQ-Mem showed bright TP-excited fluorescence (TPEF) and remarkable selectivity for the plasma membrane. Furthermore, simultaneous in vivo imaging with THQ-Mem and a TP lipid droplet probe could serve as an efficient tool to monitor morphological and physiological changes in the plasma membrane and lipid droplets.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.2c03285